Development of an electrochemical immunosensor for fumonisins detection in foods
| dc.contributor.author | Kadir, Mohamad Kamal Abdul | - |
| dc.contributor.author | Tothill, Ibtisam E. | - |
| dc.date.accessioned | 2012-05-24T23:01:15Z | |
| dc.date.available | 2012-05-24T23:01:15Z | |
| dc.date.issued | 2010-04-30T00:00:00Z | - |
| dc.description.abstract | An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L-1for fumonisins. This was then transferred to the surface of a bare gold screen-printed electrode (SPGE) and detection was performed by chronoamperometry, monitoring the reaction of 3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2) catalysed by HRP at -100 mV potential vs. onboard Ag-AgCl pseudo-reference electrode. The immunosensor exhibited detection limit of 5 µg·L-1 fumonisins with a dynamic range from 1 µg·L-1-1000 µg·L-1. The sensor also performed well in extracted corn | en_UK |
| dc.identifier.citation | Mohamad K.A. Kadir and I.E. Tothill. Development of an electrochemical immunosensor for fumonisins detection in foods. Toxins, 2010, Volume 2, Issue 4, pp382-398. | |
| dc.identifier.issn | 2072-6651 | - |
| dc.identifier.uri | http://dx.doi.org/10.3390/toxins2040382 | - |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/7177 | |
| dc.publisher | MDPI | en_UK |
| dc.title | Development of an electrochemical immunosensor for fumonisins detection in foods | en_UK |
| dc.type | Article | - |