Myelin basic protein peptide 45–89 induces the release of nitric oxide from microglial cells.

dc.contributor.authorShanshiashvili, L.
dc.contributor.authorPichkhadze, B.
dc.contributor.authorMachaidze, G.
dc.contributor.authorRamsden, Jeremy J.
dc.contributor.authorMikeladze, D.
dc.date.accessioned2008-05-08T10:59:09Z
dc.date.available2008-05-08T10:59:09Z
dc.date.issued2002-06
dc.description.abstractContinuous (24 h) exposure of mixed oligodendrocyte/microglial cells to peptides 45–89 derived from citrullinated C8 isoforms of myelin basic protein (MBP) induces cell death. In contrast, MBP-C8 at the same molecular concentration is not toxic to oligodendrocyte/microglial cells as detected by the MTT test and trypan blue exclusion method. The loss of oligodendrocyte/microglial cells resulted in the release of cytochrome c from mitochondria, suggesting MBP 45–89-induced apoptosis. On the other hand, peptides 45–89 stimulated the secretion of nitric oxide from microglial cells only via induction of iNOS. The addition of peptide 45–89 to the microglial cells led to a decrease of the level of the inhibitory protein IkB, indicating that activation of the transcription factor NF-kB is involved in these processes. We propose that the immunodominant peptide 45–89 induces damage of oligodendrocytes by activation of microglial cells and subsequent generation of nitric oxide, and that this may be the first step in the initiation of autoimmunity.en_UK
dc.identifier.citationL. Shanshiashvili, B. Pichkhadze, G. Machaidze, J.J. Ramsden, D. Mikeladze; Myelin basic protein peptide 45–89 induces the release of nitric oxide from microglial cells. Journal of Biological Physics and Chemistry Vol 2 No 1/2, 2002 pp7–11en_UK
dc.identifier.issn1512-0856
dc.identifier.urihttp://hdl.handle.net/1826/2534
dc.language.isoenen_UK
dc.publisherJointly by, Collegium Basilea (Institute of Advanced Study) and Association of Modern Scientific Investigation.en_UK
dc.relation.ispartofwww.amsi.ge/jbpc
dc.titleMyelin basic protein peptide 45–89 induces the release of nitric oxide from microglial cells.en_UK
dc.typeArticleen_UK

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