Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex
Date published
2010-11-15T00:00:00Z
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Elsevier Science B.V., Amsterdam.
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Article
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0308-8146
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Alfred E. Thumser, Aswir Abd Rashed, Paul A. Sharp, John K. Lodge; Ascorbate enhances iron uptake into intestinal cells through formation of a
FeCl3-ascorbate complex, Food Chemistry, Volume 123, Issue 2, 15 November 2010, Pages 281-285
Abstract
It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration- dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+-ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+-ascorbate complex. (C) 2010 Elsevier Ltd. All rights reserved.
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Enterocyte iron uptake Ascorbate Ferric iron caco-2 cells nonheme iron colorimetric assay epithelial-cells expression transport absorption dmt1 bioavailability humans