Exploiting mycorrhizal selection of beneficial rhizosphere bacteria from the soil microbiome.

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dc.contributor.advisorMauchline, Tim
dc.contributor.advisorOtten, Wilfred
dc.contributor.advisorHirsch, Penny
dc.contributor.advisorBrennan, Fiona
dc.contributor.advisorClark, Ian
dc.contributor.advisorHarris, Jim A.
dc.contributor.authorMasters-Clark, Emily
dc.date.accessioned2024-03-19T17:19:28Z
dc.date.available2024-03-19T17:19:28Z
dc.date.issued2021-09
dc.description.abstractSoil health is dependent on its diverse communities of microbes. Many of these microorganisms enhance plant growth and enrich the soil. However, the interactions between communities of beneficial microbes remain unclear. Arbuscular mycorrhizal fungi (AMF) are responsible for the most prolific beneficial plant-fungal interaction. However, their influence on the diverse range of plant growth promoting rhizobacteria (PGPR) that also associate with plant roots is yet to be fully elucidated. This research investigates the tripartite interactions between host plant-AMF-PGPR using next-generation sequencing and culture- dependent methodology to define the effect of AMF inoculation on the taxonomic and functional characteristics of the bacterial assemblage of the root microbiome of white clover (Trifolium repens). Soil from two land use types (grassland and bare fallow) amended with fertiliser and/or AMF inoculants are used to describe the effect of these management components on the function of beneficial microbes in cropping systems. The AMF Funneliformis geosporum affected the taxonomic composition of bacteria in the rhizosphere but not the rhizoplane. However, soil type and fertiliser were more influential determinants of bacterial taxa and function. Using split-root microcosm experiments with root exclusion meshes, the dispersal of bacteria was observed in the absence of AMF hyphae. The approaches were combined to show that root microbiome establishment is independent of AMF hyphal facilitation or selection of beneficial bacterial traits or taxa. In vitro predictive measures were used to design a putative Phosphorus solubilising consortium comprised of synergistic P-solubilising rhizobacteria and AMF. Plant health parameters were influenced by the addition of Ca₃PO₄ but were unaffected by any microbial combination. The performance of a putative bioinoculant is dependent on many external factors which can negatively impact the intended function. This work is an important indicator of the complexity of the soil microbiome and demonstrates the profound influence of agronomic inputs on microbial function.en_UK
dc.description.coursenamePhD in Environment and Agrifooden_UK
dc.description.notesHarris, Jim (Associate)
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/21048
dc.language.isoen_UKen_UK
dc.publisherCranfield Universityen_UK
dc.publisher.departmentSWEEen_UK
dc.rights© Cranfield University, 2021. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder.en_UK
dc.subjectPlant-growth promoting rhizobacteriaen_UK
dc.subjectarbuscular mycorrhizal fungien_UK
dc.subjectTrifolium repensen_UK
dc.subjectamplicon sequencingen_UK
dc.subjectin vitro cultureen_UK
dc.subjectmicrobial interactionsen_UK
dc.subjectmicrobial bioinoculantsen_UK
dc.subjectplant nutrient contenten_UK
dc.subjectPhosphorus solubilising microorganismsen_UK
dc.subjectsustainable agricultureen_UK
dc.titleExploiting mycorrhizal selection of beneficial rhizosphere bacteria from the soil microbiome.en_UK
dc.typeThesis or dissertationen_UK
dc.type.qualificationlevelDoctoralen_UK
dc.type.qualificationnamePhDen_UK

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