Seed dormancy and germination responses of cannabis landraces to various pre-treatments

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dc.contributor.authorLanga, Sabeliwe
dc.contributor.authorMagwaza, Lembe Samukelo
dc.contributor.authorMditshwa, Asanda
dc.contributor.authorTesfay, Samson Zeray
dc.date.accessioned2024-02-12T11:36:25Z
dc.date.available2024-02-12T11:36:25Z
dc.date.issued2023-12-22
dc.description.abstractThe current study evaluated the effectiveness of different pre-treatments for dormancy breaking and seed germination of five cannabis landraces. These landraces were collected from local growers in three regions of KwaZulu-Natal Province in South Africa, namely, Bergville (B), Hammersdale (H), and Ladysmith (L). Each genotype was assigned an alphabet and number based on where the seed material was collected. The five landraces were as follows, ‘B1’, ‘K1’, ‘H1’, ‘L1’, and ‘L2’. The study was conducted using various pre-treatments, including potassium nitrate (KNO3), hydrochloric acid (HCL), hot water (HW) at 70 °C, nitric acid (HNO3), dry prechilling at 10 °C, gibberellic acid (GA3), sulfuric acid (H2SO4), and water, which served as the control. Before the germination test, the seed viability of cannabis landraces was assessed using the tetrazolium chloride (TCC) test. Afterward, the seeds were germinated in Petri dishes with cotton pads moistened with distilled water for five (5) days. The study was later validated under tunnel conditions. The parameters such as germination percentage, germination rate, seedling length, and seed vigour index of the germinated seeds were then measured. The landraces, dormancy breaking treatments, and their interactions showed significant differences (p < 0.001) in germination percentage, germination rate index, seedling length, and seed vigour index under both laboratory and tunnel conditions. In summary, GA3, KNO3 and prechilling were the most effective pre-treatments to improve the germination and seedling growth of cannabis genotypes and breaking seed dormancy. Therefore, it was concluded that cannabis genotypes had physiological dormancy.en_UK
dc.identifier.citationLanga S, Magwaza LS, Mditshwa A, Tesfay SZ. (2024) Seed dormancy and germination responses of cannabis landraces to various pre-treatments. South African Journal of Botany, Volume 165, February 2024, pp.91-100en_UK
dc.identifier.eissn1727-9321
dc.identifier.issn0254-6299
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2023.12.021
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/20775
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCannabisen_UK
dc.subjectDormancy breakingen_UK
dc.subjectGermination enhancementen_UK
dc.subjectLandraceen_UK
dc.titleSeed dormancy and germination responses of cannabis landraces to various pre-treatmentsen_UK
dc.typeArticleen_UK
dcterms.dateAccepted2023-12-13

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