Magnesium supply alleviates iron toxicity-induced leaf bronzing in rice through exclusion and tissue-tolerance mechanisms

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dc.contributor.authorRajonandraina, Toavintsoa
dc.contributor.authorUeda, Yoshiaki
dc.contributor.authorWissuwa, Matthias
dc.contributor.authorKirk, Guy J. D.
dc.contributor.authorRakotoson, Tovohery
dc.contributor.authorManwaring, Hanna
dc.contributor.authorAndriamananjara, Andry
dc.contributor.authorRazafimbelo, Tantely
dc.date.accessioned2023-08-08T12:58:03Z
dc.date.available2023-08-08T12:58:03Z
dc.date.issued2023-07-21
dc.description.abstractIntroduction: Iron (Fe) toxicity is a widespread nutritional disorder in lowland rice causing growth retardation and leaf symptoms referred to as leaf bronzing. It is partly caused by an imbalance of nutrients other than Fe and supply of these is known to mitigate the toxicity. But the physiological and molecular mechanisms involved are unknown. Methods: We investigated the effect of magnesium (Mg) on Fe toxicity tolerance in a field study in the Central Highlands of Madagascar and in hydroponic experiments with excess Fe (300 mg Fe L-1). An RNA-seq analysis was conducted in a hydroponic experiment to elucidate possible mechanisms underlying Mg effects. Results and discussion: Addition of Mg consistently decreased leaf bronzing under both field and hydroponic conditions, whereas potassium (K) addition caused minor effects. Plants treated with Mg tended to have smaller shoot Fe concentrations in the field, suggesting enhanced exclusion at the whole-plant level. However, analysis of multiple genotypes showed that Fe toxicity symptoms were also mitigated without a concomitant decrease of Fe concentration, suggesting that increased Mg supply confers tolerance at the tissue level. The hydroponic experiments also suggested that Mg mitigated leaf bronzing without significantly decreasing Fe concentration or oxidative stress as assessed by the content of malondialdehyde, a biomarker for oxidative stress. An RNA-seq analysis revealed that Mg induced more changes in leaves than roots. Subsequent cis-element analysis suggested that NAC transcription factor binding sites were enriched in genes induced by Fe toxicity in leaves. Addition of Mg caused non-significant enrichment of the same binding sites, suggesting that NAC family proteins may mediate the effect of Mg. This study provides clues for mitigating Fe toxicity-induced leaf bronzing in rice.en_UK
dc.identifier.citationRajonandraina T, Ueda Y, Wissuwa M, et al., (2023) Magnesium supply alleviates iron toxicity-induced leaf bronzing in rice through exclusion and tissue-tolerance mechanisms. Frontiers in Plant Science, Volume 14, July 2023, Article No. 1213456en_UK
dc.identifier.issn1664-462X
dc.identifier.urihttps://doi.org/10.3389/fpls.2023.1213456
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/20062
dc.language.isoenen_UK
dc.publisherFrontiersen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectriceen_UK
dc.subjectiron toxicityen_UK
dc.subjectmagnesiumen_UK
dc.subjectleaf bronzingen_UK
dc.subjectfertilizeren_UK
dc.subjectRNA-Seqen_UK
dc.titleMagnesium supply alleviates iron toxicity-induced leaf bronzing in rice through exclusion and tissue-tolerance mechanismsen_UK
dc.typeArticleen_UK

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