Tunable 3D nanofibrous and bio-functionalised PEDOT network explored as a conducting polymer-based biosensor

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dc.contributor.authorMeng, Lingyin
dc.contributor.authorTurner, Anthony P. F.
dc.contributor.authorMak, Wing Cheung
dc.date.accessioned2020-11-05T13:03:48Z
dc.date.available2020-11-05T13:03:48Z
dc.date.issued2020-04-01
dc.description.abstractConducting polymers that possess good electrochemical properties, nanostructured morphology and functionality for bioconjugation are essential to realise the concept of all-polymer-based biosensors that do not depend on traditional nanocatalysts such as carbon materials, metal, metal oxides or dyes. In this research, we demonstrated a facile approach for the simultaneous preparation of a bi-functional PEDOT interface with a tunable 3D nanofibrous network and carboxylic acid groups (i.e. Nano-PEDOT-COOH) via controlled co-polymerisation of EDOT and EDOT-COOH monomers, using tetrabutylammonium perchlorate as a soft-template. By tuning the ratio between EDOT and EDOT-COOH monomer, the nanofibrous structure and carboxylic acid functionalisation of Nano-PEDOT-COOH were varied over a fibre diameter range of 15.6 ± 3.7 to 70.0 ± 9.5 nm and a carboxylic acid group density from 0.03 to 0.18 μmol cm−2. The nanofibres assembled into a three-dimensional network with a high specific surface area, which contributed to low charge transfer resistance and high transduction activity towards the co-enzyme NADH, delivering a wide linear range of 20–960 μM and a high sensitivity of 0.224 μA μM−1 cm−2 at the Nano-PEDOT-COOH50% interface. Furthermore, the carboxylic acid groups provide an anchoring site for the stable immobilisation of an NADH-dependent dehydrogenase (i.e. lactate dehydrogenase), via EDC/S–NHS chemistry, for the fabrication of a Bio-Nano-PEDOT-based biosensor for lactate detection which had a response time of less than 10 s over the range of 0.05–1.8 mM. Our developed bio-Nano-PEDOT interface shows future potential for coupling with multi-biorecognition molecules via carboxylic acid groups for the development of a range of advanced all-polymer biosensorsen_UK
dc.identifier.citationMeng L, Turner APF, Cheung-Mak W. (2020) Tunable 3D nanofibrous and bio-functionalised PEDOT network explored as a conducting polymer-based biosensor. Biosensors and Bioelectronics, Volume 159, July 2020, Article number 112181en_UK
dc.identifier.issn0956-5663
dc.identifier.urihttps://doi.org/10.1016/j.bios.2020.112181
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/15964
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectConducting polymeren_UK
dc.subjectNanofibreen_UK
dc.subjectCarboxylic acid functionalisationen_UK
dc.subjectBio-nano-PEDOTen_UK
dc.titleTunable 3D nanofibrous and bio-functionalised PEDOT network explored as a conducting polymer-based biosensoren_UK
dc.typeArticleen_UK

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