The impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagic Escherichia coli

Simple item page
dc.contributor.authorCazzola, Hélène
dc.contributor.authorLemaire, Laurine
dc.contributor.authorAcket, Sébastien
dc.contributor.authorProst, Elise
dc.contributor.authorDuma, Luminita
dc.contributor.authorErhardt, Marc
dc.contributor.authorČechová, Petra
dc.contributor.authorTrouillas, Patrick
dc.contributor.authorMohareb, Fady
dc.contributor.authorRossi, Claire
dc.contributor.authorRossez, Yannick
dc.date.accessioned2020-09-23T14:08:47Z
dc.date.available2020-09-23T14:08:47Z
dc.date.issued2020-09-16
dc.description.abstractEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of foodborne gastrointestinal illness. The adhesion of EHEC to host tissues is the first step enabling bacterial colonization. Adhesins such as fimbriae and flagella mediate this process. Here, we studied the interaction of the bacterial flagellum with the host cell’s plasma membrane using giant unilamellar vesicles (GUVs) as a biologically relevant model. Cultured cell lines contain many different molecular components, including proteins and glycoproteins. In contrast, with GUVs, we can characterize the bacterial mode of interaction solely with a defined lipid part of the cell membrane. Bacterial adhesion on GUVs was dependent on the presence of the flagellar filament and its motility. By testing different phospholipid head groups, the nature of the fatty acid chains, or the liposome curvature, we found that lipid packing is a key parameter to enable bacterial adhesion. Using HT-29 cells grown in the presence of polyunsaturated fatty acid (α-linolenic acid) or saturated fatty acid (palmitic acid), we found that α-linolenic acid reduced adhesion of wild-type EHEC but not of a nonflagellated mutant. Finally, our results reveal that the presence of flagella is advantageous for the bacteria to bind to lipid rafts. We speculate that polyunsaturated fatty acids prevent flagellar adhesion on membrane bilayers and play a clear role for optimal host colonization. Flagellum-mediated adhesion to plasma membranes has broad implications for host-pathogen interactions.en_UK
dc.identifier.citationCazzola H, Lemaire L, Acket S, Prost E, Duma L, Erhardt M, Čechová P, Trouillas P, Mohareb F, Rossi C & Rossez Y (2020) The impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagic Escherichia coli. mSphere, Volume 5, Article number e00702-20en_UK
dc.identifier.issn2379-5042
dc.identifier.urihttps://doi.org/10.1128/mSphere.00702-20
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/15840
dc.language.isoenen_UK
dc.publisherAmerican Society for Microbiologyen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectadhesinsen_UK
dc.subjectflagellaen_UK
dc.subjectlipid raftsen_UK
dc.subjectphospholipidsen_UK
dc.titleThe impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagic Escherichia colien_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Impact_o_plasma_membrane_lipid_composition-2020.pdf
Size:
3.39 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SWEE)