Entropy-driven three-dimensional DNA nanofireworks for simultaneous real-time imaging of telomerase and microRNA in living cells

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dc.contributor.authorWang, Jin
dc.contributor.authorWang, Kaixuan
dc.contributor.authorPeng, Hanyong
dc.contributor.authorZhang, Zhen
dc.contributor.authorYang, Zhugen
dc.contributor.authorSong, Maoyong
dc.contributor.authorJiang, Guibin
dc.date.accessioned2023-02-20T09:44:14Z
dc.date.available2023-02-20T09:44:14Z
dc.date.issued2023-02-15
dc.description.abstractReal-time monitoring of different types of intracellular tumor-related biomarkers is of key importance for the identification of tumor cells. However, it is hampered by the low abundance of biomarkers, inefficient free diffusion of reactants, and complex cytoplasmic milieu. Herein, we present a stable and general method for in situ imaging of microRNA-21 and telomerase utilizing simple highly integrated dual tetrahedral DNA nanostructures (TDNs) that can naturally enter cells, which could initiate to form the three-dimensional (3D) higher-order DNA superstructures (DNA nanofireworks, DNFs) through a reliable target-triggered entropy-driven strand displacement reaction in living cells for remarkable signal amplification. Importantly, the excellent biostability, biocompatibility, and sensitivity of this approach benefited from (i) the precise multidirectional arrangement of probes with a pure DNA structure and (ii) the local target concentration enhanced by the spatially confined microdomain inside the DNFs. This strategy provides a pivotal molecular toolbox for broad applications such as biomedical imaging and early precise cancer diagnosis.en_UK
dc.identifier.citationWang J, Wang K, Peng H, et al., (2023) Entropy-driven three-dimensional DNA nanofireworks for simultaneous real-time imaging of telomerase and microRNA in living cells. Analytical Chemistry, Volume 95, Issue 8, February 2023, pp. 4138-4146en_UK
dc.identifier.issn0003-2700
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.2c05200
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/19212
dc.language.isoenen_UK
dc.publisherAmerican Chemical Societyen_UK
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectReal-time monitoringen_UK
dc.subjectSignal amplificationen_UK
dc.subjectBiosensoren_UK
dc.subjectTetrahedral DNA nanostructureen_UK
dc.subjectTelomeraseen_UK
dc.subjectMicroRNAen_UK
dc.titleEntropy-driven three-dimensional DNA nanofireworks for simultaneous real-time imaging of telomerase and microRNA in living cellsen_UK
dc.typeArticleen_UK

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