International interlaboratory study to normalize liquid chromatography-based mycotoxin retention times through implementation of a retention index system

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dc.contributor.authorKelman, Megan J.
dc.contributor.authorRenaud, Justin B.
dc.contributor.authorMcCarron, Pearse
dc.contributor.authorHoogstra, Shawn
dc.contributor.authorChow, Willis
dc.contributor.authorWang, Jian
dc.contributor.authorVarga, Liz
dc.contributor.authorPatriarca, Andrea
dc.contributor.authorMedina, Angel
dc.contributor.authorVisintin, Lia
dc.contributor.authorNguyen, Truong Nhat
dc.contributor.authorDe Boevre, Marthe
dc.contributor.authorDe Saeger, Sarah
dc.contributor.authorKaranghat, Vasudevan
dc.contributor.authorVuckovic, Dajana
dc.contributor.authorMcMullin, David R.
dc.contributor.authorDall'Asta, Chiara
dc.contributor.authorAyeni, Kolawole I.
dc.contributor.authorWarth, Benedikt
dc.contributor.authorHuang, Mei
dc.contributor.authorTittlemier, Sheryl A.
dc.contributor.authorMats, Lili
dc.contributor.authorCao, Rong
dc.contributor.authorSulyok, Michael
dc.contributor.authorXu, Kangkang
dc.contributor.authorBerthiller, Franz
dc.contributor.authorKuhn, Michael
dc.contributor.authorCramer, Benedikt
dc.contributor.authorCiasca, Biancamaria
dc.contributor.authorLattanzio, Veronica M. T.
dc.contributor.authorDe Baere, Siegrid
dc.contributor.authorCroubels, Siska
dc.contributor.authorDesRochers, Natasha
dc.contributor.authorSura, Srinivas
dc.contributor.authorBates, Jennifer
dc.contributor.authorWright, Elliott James
dc.contributor.authorThapa, Indira
dc.contributor.authorBlackwell, Barbara A.
dc.contributor.authorZhang, Kai
dc.contributor.authorWong, Jon W.
dc.contributor.authorBurns, Laura
dc.contributor.authorBorts, David J.
dc.contributor.authorSumarah, Mark W.
dc.date.accessioned2025-03-13T14:39:30Z
dc.date.available2025-03-13T14:39:30Z
dc.date.freetoread2025-03-13
dc.date.issued2025-03-29
dc.date.pubOnline2025-02-05
dc.description.abstractMonitoring for mycotoxins in food or feed matrices is necessary to ensure the safety and security of global food systems. Due to a lack of standardized methods and individual laboratory priorities, most institutions have developed their own methods for mycotoxin determinations. Given the diversity of mycotoxin chemical structures and physicochemical properties, searching databases, and comparing data between institutions is complicated. We previously introduced incorporating a retention index (RI) system into liquid chromatography mass spectrometry (LC-MS) based mycotoxin determinations. To validate this concept, we designed an interlaboratory study where each participating laboratory was sent N-alkylpyridinium-3-sulfonates (NAPS) RI standards, and 36 mycotoxin standards for analysis using their pre-optimized LC-MS methods. Data from 44 analytical methods were submitted from 24 laboratories representing various manufacturer platforms, LC columns, and mobile phase compositions. Mycotoxin retention times (tR) were converted to RI values based on their elution relative to the NAPS standards. Trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol) showed tR consistency (± 20–50 RI units, 1–5 % median RI) regardless of mobile phase or type of chromatography column in this study. For the remaining mycotoxins tested, the RI values were strongly impacted by the mobile phase composition and column chemistry. The ability to predict tR was evaluated based on the median RI mycotoxin values and the NAPS tR. These values were corrected using Tanimoto coefficients to investigate whether structurally similar compounds could be used as anchors to further improve accuracy. This study demonstrated the power of employing an RI system for mycotoxin determinations, further enhancing the confidence of identifications.
dc.description.journalNameJournal of Chromatography A
dc.description.sponsorshipGenome Canada, FWF Austrian Science Fund, Agriculture and Agri-Food Canada, Ministry of Education, Universities and Research, National Research Council Canada, Mitacs
dc.description.sponsorshipThis research was supported by the NRC (Biotoxin Metrology, Nova Scotia), the ALIFAR project (Italian Ministry of University, Dipartimenti di Eccellenza 2023–2027), Genome Canada Technology Development Grant and MITACS scholarship, with resources provided by the VetCore Facility (Mass Spectrometry) of the University of Veterinary Medicine Vienna.
dc.description.sponsorshipMoreover, this research was supported by the Austrian Science Fund (FWF, P33188), the Mass Spectrometry Centre of the Faculty of Chemistry and the Exposome Austria Research Infrastructure at the University of Vienna.
dc.format.mediumPrint-Electronic
dc.identifier.citationKelman MJ, Renaud JB, McCarron P, et al., (2025) International interlaboratory study to normalize liquid chromatography-based mycotoxin retention times through implementation of a retention index system. Journal of Chromatography A, Volume 1745, March 2025, Article number 465732en_UK
dc.identifier.eissn1873-3778
dc.identifier.elementsID563974
dc.identifier.issn0021-9673
dc.identifier.paperNo465732
dc.identifier.urihttps://doi.org/10.1016/j.chroma.2025.465732
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23599
dc.identifier.volumeNo1745
dc.languageEnglish
dc.language.isoen
dc.publisherElsevieren_UK
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0021967325000810?via%3Dihub
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBelgiumen_UK
dc.subjectReverse phase chromatographyen_UK
dc.subjectLC-MSen_UK
dc.subjectMycotoxin determinationen_UK
dc.subjectTanimoto structural similarity coefficienten_UK
dc.subjectN-alkylpyridinium-3-sulfonatesen_UK
dc.subject3401 Analytical Chemistryen_UK
dc.subject34 Chemical Sciencesen_UK
dc.subject40 Engineeringen_UK
dc.subject.meshMycotoxinsen_UK
dc.subject.meshChromatography, Liquiden_UK
dc.subject.meshLaboratoriesen_UK
dc.subject.meshMass Spectrometryen_UK
dc.subject.meshFood Contaminationen_UK
dc.subject.meshTrichothecenesen_UK
dc.subject.meshTandem Mass Spectrometryen_UK
dc.subject.meshPyridinium Compoundsen_UK
dc.titleInternational interlaboratory study to normalize liquid chromatography-based mycotoxin retention times through implementation of a retention index systemen_UK
dc.typeArticle
dc.type.subtypeJournal Article
dcterms.dateAccepted2025-01-27

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