Rapid enzymatic assays for fecal contamination in aquatic environment: challenges, advances and prospects

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dc.contributor.authorYuan, Xiaofei
dc.contributor.authorGlidle, Andrew
dc.contributor.authorYang, Zhugen
dc.contributor.authorWang, Baojun
dc.date.accessioned2024-05-23T12:54:50Z
dc.date.available2024-05-23T12:54:50Z
dc.date.issued2024-05-22
dc.description.abstractRoutine monitoring of sanitation and hygiene to identify fecal contamination in aquatic environments is an effective means to prevent threatening disease transmission. Compared to immunological or genetic methods performed in a central lab, enzymatic assays are considered simple, quick, cost-effective and thus promising for in-field (near) real-time detection. However, the long detection time for mildly polluted samples is a major obstacle to its deployment as an early warning system. Here, the challenges faced by the assays in real environmental sample measurements are summarized, followed by the current status of their field applications. Furthermore, the likelihood and ways are discussed for significant assay improvements using state-of-the-art synthetic biology technologies. Rapid advances in synthetic biology such as various new enabling tools for precise biomolecular manipulation and cell-free expression systems have great potential to address the present bottlenecks of the enzymatic assays, paving the way for better early warning strategies and performance.en_UK
dc.identifier.citationYuan X, Glidle A, Yang Z, Wang B. (2024) Rapid enzymatic assays for fecal contamination in aquatic environment: challenges, advances and prospects. TrAC Trends in Analytical Chemistry Volume 176, July 2024, Article number 117768en_UK
dc.identifier.eissn1879-3142
dc.identifier.issn0165-9936
dc.identifier.urihttps://doi.org/10.1016/j.trac.2024.117768
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/21658
dc.language.isoen_UKen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectRapid enzymatic assaysen_UK
dc.subjectfecal contaminationen_UK
dc.subjectE. colien_UK
dc.subjecttotal coliformen_UK
dc.subjectsynthetic biologyen_UK
dc.subjectfield applicationen_UK
dc.subjectaquatic environmenten_UK
dc.subjectb-D-galactosidaseen_UK
dc.subjectb-D-glucuronidaseen_UK
dc.titleRapid enzymatic assays for fecal contamination in aquatic environment: challenges, advances and prospectsen_UK
dc.typeArticleen_UK
dcterms.dateAccepted2024-05-15

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