Efficacy of fungal and bacterial antagonists for controlling growth, FUM1 gene expression and fumonisin B 1 production by Fusarium verticillioides on maize cobs of different ripening stages

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dc.contributor.authorSamsudin, Nik Iskandar Putra
dc.contributor.authorRodriguez Sixtos Higuera, Alicia
dc.contributor.authorMedina-Vayá, Ángel
dc.contributor.authorMagan, Naresh
dc.date.accessioned2017-03-17T14:20:08Z
dc.date.available2017-03-17T14:20:08Z
dc.date.issued2017-02-09
dc.description.abstractThis study was carried out to examine the efficacy of two biocontrol agents (Clonostachys rosea 016, BCA1; Gram-negative bacterium, BCA5) for control of FUM1 gene expression and fumonisin B1 (FB1) production by F. verticillioides FV1 on maize cobs of different ripening stages: R3, Milk (0.985 aw); R4, Dough (0.976 aw); R5, Dent (0.958 aw). Initially, temporal studies on FUM1 gene expression and FB1 production were performed on maize kernels for up to 14 days. This revealed that day 10 was optimum for both parameters, and was used in the biocontrol studies. Maize cobs were inoculated with 50:50 mixtures of the pathogen:antagonist inoculum and incubated in environmental chambers to maintain the natural aw conditions for ten days at 25 and 30 °C. The growth rates of F. verticillioides FV1, the relative expression of the FUM1 gene and FB1 production were quantified. It was found that, aw × temp had significant impacts on growth, FUM1 gene expression and FB1 production by F. verticillioides FV1 on maize cobs of different maturities. The fungal antagonist (BCA1) significantly reduced FB1 contamination on maize cobs by > 70% at 25 °C, and almost 60% at 30 °C regardless of maize ripening stage. For the bacterial antagonist (BCA5) however, FB1 levels on maize cobs were significantly decreased only in some treatments. These results suggest that efficacy of antagonists to control mycotoxin production in ripening maize cobs needs to take account of the ecophysiology of the pathogen and the antagonists, as well as the physiological status of the maize during silking to ensure effective control.en_UK
dc.identifier.citationSamsudin NI, Rodriguez A, Medina A, Magan N, Efficacy of fungal and bacterial antagonists for controlling growth, FUM1 gene expression and fumonisin B 1 production by Fusarium verticillioides on maize cobs of different ripening stages, International Journal of Food Microbiology, Volume 246, 4 April 2017, Pages 72 – 79.en_UK
dc.identifier.issn0168-1605
dc.identifier.urihttp://dx.doi.org/10.1016/j.ijfoodmicro.2017.02.004
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/11615
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBiocontrolen_UK
dc.subjectFusariumen_UK
dc.subjectFumonisin B1en_UK
dc.subjectFUM genesen_UK
dc.subjectMaize coben_UK
dc.subjectMaturity stagesen_UK
dc.subjectSilkingen_UK
dc.titleEfficacy of fungal and bacterial antagonists for controlling growth, FUM1 gene expression and fumonisin B 1 production by Fusarium verticillioides on maize cobs of different ripening stagesen_UK
dc.typeArticleen_UK

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