Microbial degradation of Cold Lake Blend and Western Canadian select dilbits by freshwater enrichments

Simple item page
dc.contributor.authorDeshpande, Ruta S.
dc.contributor.authorSundaravadivelu, Devi
dc.contributor.authorTechtmann, Stephen
dc.contributor.authorConmy, Robyn N.
dc.contributor.authorSanto Domingo, Jorge W.
dc.contributor.authorCampo, Pablo
dc.date.accessioned2018-04-20T09:52:34Z
dc.date.available2018-04-20T09:52:34Z
dc.date.issued2018-03-21
dc.description.abstractTreatability experiments were conducted to determine the biodegradation of diluted bitumen (dilbit) at 5 and 25 °C for 72 and 60 days, respectively. Microbial consortia obtained from the Kalamazoo River Enbridge Energy spill site were enriched on dilbit at both 5 (cryo) and 25 (meso) ºC. On every sampling day, triplicates were sacrificed and residual hydrocarbon concentrations (alkanes and polycyclic aromatic hydrocarbons) were determined by GCMS/MS. The composition and relative abundance of different bacterial groups were identified by 16S rRNA gene sequencing analysis. While some physicochemical differences were observed between the two dilbits, their biodegradation profiles were similar. The rates and extent of degradation were greater at 25 °C. Both consortia metabolized 99.9% of alkanes; however, the meso consortium was more effective at removing aromatics than the cryo consortium (97.5 vs 70%). Known hydrocarbon-degrading bacteria were present in both consortia (Pseudomonas, Rhodococcus, Hydrogenophaga, Parvibaculum, Arthrobacter, Acidovorax), although their relative abundances depended on the temperatures at which they were enriched. Regardless of the dilbit type, the microbial community structure significantly changed as a response to the diminishing hydrocarbon load. Our results demonstrate that dilbit can be effectively degraded by autochthonous microbial consortia from sites with recent exposure to dilbit contamination.en_UK
dc.identifier.citationDeshpande RS, Sundaravadivelu D, Techmann S, Conmy RN, Santo Domingo JW, Campo P, Microbial degradation of Cold Lake blend and Western Canadian select dilbits by freshwater enrichments, Journal of Hazardous Materials, Vol. 352, 15 June 2018, pp. 111-120en_UK
dc.identifier.cris19997300
dc.identifier.issn0304-3894
dc.identifier.urihttp://dx.doi.org/10.1016/j.jhazmat.2018.03.030
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/13159
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectNon-conventional oilen_UK
dc.subjectDiluted bitumenen_UK
dc.subjectDilbiten_UK
dc.subjectBiodegradationen_UK
dc.subjectMicrobial community structureen_UK
dc.titleMicrobial degradation of Cold Lake Blend and Western Canadian select dilbits by freshwater enrichmentsen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Microbial_degradation_of_cold_lake_blend-2018.pdf
Size:
2.1 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SWEE)