Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays

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dc.contributor.authorPiletsky, Sergey A.-
dc.contributor.authorPiletska, Elena V.-
dc.contributor.authorBossi, Alessandra-
dc.contributor.authorKarim, K.-
dc.contributor.authorLowe, P.-
dc.contributor.authorTurner, Anthony P. F.-
dc.date.accessioned2012-02-03T23:08:42Z
dc.date.available2012-02-03T23:08:42Z
dc.date.issued2001-12-31T00:00:00Z-
dc.description.abstractA new technique for coating microtitre plates with molecularly imprinted polymers (MIP), specific for low-molecular weight analytes (epinephrine, atrazine) and proteins is presented. Oxidative polymerization was performed in the presence of template; monomers: 3-aminophenylboronic acid, 3- thiopheneboronic acid and aniline were polymerized in water and the polymers were grafted onto the polystyrene surface of the microplates. It was found that this process results in the creation of synthetic materials with antibody-like binding properties. It was shown that the MIP-coated microplates are particularly useful for assay development. The high stability of the polymers and good reproducibility of the measurements make MIP coating an attractive alternative to conventional antibodies or receptors used in ELISA.en_UK
dc.identifier.citationSergey A. Piletsky, Elena V. Piletska, Alessandra Bossi, Khalku Karim, Philip Lowe and Anthony P. F. Turner, Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays, Biosensors and Bioelectronics, Volume 16, Issues 9-12, December 2001, Pages 701- 707-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://dx.doi.org/10.1016/S0956-5663(01)00234-2-
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/802
dc.language.isoen_UK-
dc.publisherElsevier Science B.V., Amsterdam.en_UK
dc.subjectFluorescent immunoassayen_UK
dc.subjectConjugated polymersen_UK
dc.subjectGrafting on microplatesen_UK
dc.subjectMolecular imprintingen_UK
dc.titleSubstitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assaysen_UK
dc.typeArticle-

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