An SPR based sensor for allergens detection

dc.contributor.authorAshley, J.
dc.contributor.authorPiekarska, M.
dc.contributor.authorSegers, C.
dc.contributor.authorTrinh, L.
dc.contributor.authorRodgers, T.
dc.contributor.authorWilley, R.
dc.contributor.authorTothill, Ibtisam E.
dc.date.accessioned2016-08-05T13:44:13Z
dc.date.available2016-08-05T13:44:13Z
dc.date.issued2016-07-28
dc.description.abstractA simple, sensitive and label-free optical sensor method was developed for allergens analysis using α-casein as the biomarker for cow's milk detection, to be used directly in final rinse samples of cleaning in place systems (CIP) of food manufacturers. A Surface Plasmon Resonance (SPR) sensor chip consisting of four sensing arrays enabling the measurement of samples and control binding events simultaneously on the sensor surface was employed in this work. SPR offers several advantages in terms of label free detection, real time measurements and superior sensitivity when compared to ELISA based techniques. The gold sensor chip was used to immobilise α-casein-polyclonal antibody using EDC/NHS coupling procedure. The performance of the assay and the sensor was first optimised and characterised in pure buffer conditions giving a detection limit of 58 ng mL−1 as a direct binding assay. The assay sensitivity can be further improved by using sandwich assay format and amplified with nanoparticles. However, at this stage this is not required as the detection limit achieved exceeded the required allergens detection levels of 2 µg mL−1 for α-S1-casein. The sensor demonstrated good selectivity towards the α-casein as the target analyte and adequate recoveries from CIP final rinse wash samples. The sensor would be useful tool for monitoring allergen levels after cleaning procedures, providing additional data that may better inform upon wider food allergen risk management decision(s) that are made by food manufacturer. In particular, this sensor could potentially help validate or optimise cleaning practices for a given food manufacturing process.en_UK
dc.identifier.citationJ. Ashley, M. Piekarska, C. Segers, L. Trinh, T. Rodgers, R. Willey, I.E. Tothill, An SPR based sensor for allergens detection, Biosensors and Bioelectronics, Volume 88, 15 February 2017, Pages 109–113en_UK
dc.identifier.issn0956-5663
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2016.07.101.
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/10238
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-Non-Commercial-No Derivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSurface plasmon resonanceen_UK
dc.subjectSPRen_UK
dc.subjectMilk proteinsen_UK
dc.subjectAllergensen_UK
dc.subjectCleaning in place systemsen_UK
dc.subjectCIPen_UK
dc.subjectAntibodyen_UK
dc.titleAn SPR based sensor for allergens detectionen_UK
dc.typeArticleen_UK

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