Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer

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dc.contributor.authorBouzy, Pascaline
dc.contributor.authorLyburn, Iain Douglas
dc.contributor.authorPinder, Sarah E.
dc.contributor.authorScott, Robert
dc.contributor.authorMansfield, Jessica
dc.contributor.authorMoger, Julian
dc.contributor.authorGreenwood, Charlene
dc.contributor.authorBouybayoune, Ihssane
dc.contributor.authorCornford, Eleanor
dc.contributor.authorRogers, Keith
dc.contributor.authorStone, Nick
dc.date.accessioned2023-03-24T16:19:27Z
dc.date.available2023-03-24T16:19:27Z
dc.date.issued2023-02-21
dc.description.abstractMicrocalcifications play an important role in cancer detection. They are evaluated by their radiological and histological characteristics but it is challenging to find a link between their morphology, their composition and the nature of a specific type of breast lesion. Whilst there are some mammographic features that are either typically benign or typically malignant often the appearances are indeterminate. Here, we explore a large range of vibrational spectroscopic and multiphoton imaging techniques in order to gain more information about the composition of the microcalcifications. For the first time, we validated the presence of carbonate ions in the microcalcifications by O-PTIR and Raman spectroscopy at the same time, the same location and the same high resolution (0.5 μm). Furthermore, the use of multiphoton imaging allowed us to create stimulated Raman histology (SRH) images which mimic histological images with all chemical information. In conclusion, we established a protocol for efficiently analysing the microcalcifications by iteratively refining the area of interest.en_UK
dc.identifier.citationBouzy P, Lyburn ID, Pinder SE, et al., (2023) Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer. Analytical Methods, Volume 15, Issue 13, 7 April 2023, pp. 1620–1630en_UK
dc.identifier.issn1759-9660
dc.identifier.urihttps://doi.org/10.1039/D2AY01197B
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/19355
dc.language.isoenen_UK
dc.publisherRoyal Society of Chemistryen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleExploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast canceren_UK
dc.typeArticleen_UK

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