Understanding the genetic and morphological basis of bushy root and bifuricate, two mutations affecting plant architecture in Solanum lycopersicum L.

Simple item page
dc.contributor.advisorThompson, Andrew J.
dc.contributor.advisorMohareb, Fady R.
dc.contributor.authorSilva Ferreira, Demetryus
dc.date.accessioned2017-08-16T11:17:11Z
dc.date.available2017-08-16T11:17:11Z
dc.date.issued2017-03
dc.description.abstractThe classical ethyl methanesulfonate (EMS) tomato mutant bushy root (brt) was studied using a homozygous near isogenic line (brtNIL) in the Micro-Tom (MT) genetic background. The mutation has a pleiotropic phenotype comprising slow seedling development, which may be a consequence of a maternally-inherited small seed phenotype, and a more compact, smaller but not bushier, root phenotype. The number of lateral roots, total root length and taproot size are all smaller in brtNIL than the WT. The BRT locus was mapped to a 137 kbp region containing 9 candidate genes on chr 12; an InDel in the promoter region of Solyc12g014590 – containing two highly conserved pirin domains (Pirin_C and Pirin), was detected. Different expression patterns were confirmed by transcriptomic results, supporting Solyc12g014590 as the gene responsible for the brt phenotype. A naturally occurring recessive mutant named bifuricate (bif) shows an increase in inflorescence (truss) branching in comparison to the wild type (WT) control line, LAM183. In addition, the number of flowers per truss was 235% higher in bif plants than WT. Low temperature is known to increase truss branching, and so a four day low temperature treatment was applied and it was demonstrated that flowering increased significantly more in bif than in LAM183. The BIF locus was mapped to a 2.01 Mbp interval of chromosome 12 containing 53 genes. All coding region polymorphisms in the interval were surveyed, and two genes Solyc12g019420 (a BTB/TAZ transcription factor) and Solyc12g019460 (a MAP kinase) contained one stop codon predicted to disrupt gene function; both genes are excellent candidates for inflorescence branching control based on literature evidence. A newly developed introgression browser was used to demonstrate that the origin of the bif mutant haplotype is Solanum galapagense.en_UK
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/12322
dc.publisherCranfield Universityen_UK
dc.rights© Cranfield University, 2017. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder.en_UK
dc.subjectbranchingen_UK
dc.subjectBTB-POZen_UK
dc.subjectBTB-TAZen_UK
dc.subjectbushinessen_UK
dc.subjectEMS-mutanten_UK
dc.subjectlow temperatureen_UK
dc.subjectMAP kinaseen_UK
dc.subjectroot architectureen_UK
dc.subjectroot branchingen_UK
dc.subjectseed areaen_UK
dc.subjectSolanum galapagenseen_UK
dc.subjecttranscriptomicsen_UK
dc.subjecttruss branchingen_UK
dc.subjectUlp 1en_UK
dc.titleUnderstanding the genetic and morphological basis of bushy root and bifuricate, two mutations affecting plant architecture in Solanum lycopersicum L.en_UK
dc.typeThesis or dissertationen_UK
dc.type.qualificationlevelDoctoralen_UK
dc.type.qualificationnamePhDen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Silva_Ferreira_D_2017.pdf
Size:
4.83 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

PhD, EngD and MSc by research theses (SWEE)