Deep eutectic solvent pretreatment of water hyacinth for improved holocellulosic saccharification and fermentative co-production of xylitol and lipids using Rhodosporidium toruloides NCIM 3547

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dc.contributor.authorUmai, Ramachandran Devasena
dc.contributor.authorJacob, Samuel
dc.contributor.authorKumar, Vinod
dc.date.accessioned2022-11-23T15:22:52Z
dc.date.available2022-11-23T15:22:52Z
dc.date.issued2022-10-31
dc.description.abstractIn this study, delignification of water hyacinth (WH) using a mild ionic liquid-like chemical deep eutectic solvent (DES) synthesized using choline chloride and urea was conducted and the process parameters were optimized by Box–Behnken design (BBD)-based response surface methodology (RSM). From the results, a delignification of 64.32 ± 4.08% (w/w) was obtained under 1:12.5 (biomass:DES ratio), 4.63 h (time) and 87 °C (temperature). Further, a dilute sulphuric acid (2%, v/v) hydrolysis was carried out to destabilize the hemicellulose that resulted in 23.7 ± 0.50 g/L of xylose. Fermentation of the obtained xylose was carried out using a red oleaginous yeast, Rhodosporidium toruloides NCIM 3547, with free and Ca2+-alginate-immobilized cells for xylitol production under microaerophilic conditions and obtained yields of 4.73 ± 0.40 g/L (168 h) and 9.18 ± 0.10 g/L (packed bed reactor with a retention time of 18 h), respectively. Further, when the same fermentation was performed under aerobic conditions about 40.93 ± 0.73% lipid accumulation was observed with free cells. For saccharification, Aspergillus-niger-derived cellulase was used and this resulted in a yield of 27.45 ± 0.04 g/L of glucose. The glucose-enriched hydrolysate was supplemented for fermentation under nitrogen starved conditions from which 46.81 ± 2.60% (w/w) lipid content was obtained.en_UK
dc.identifier.citationUmai RD, Jacob S, Kumar V. (2022) Deep eutectic solvent pretreatment of water hyacinth for improved holocellulosic saccharification and fermentative co-production of xylitol and lipids using Rhodosporidium toruloides NCIM 3547, Fermentation, Volume 8, Issue 11, October 2022, Article number 591en_UK
dc.identifier.issn2311-5637
dc.identifier.urihttps://doi.org/10.3390/fermentation8110591
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/18731
dc.language.isoenen_UK
dc.publisherMDPIen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectwater hyacinthen_UK
dc.subjectdeep eutectic solventen_UK
dc.subjectdelignificationen_UK
dc.subjectxyloseen_UK
dc.subjectxylitolen_UK
dc.subjectlipidsen_UK
dc.titleDeep eutectic solvent pretreatment of water hyacinth for improved holocellulosic saccharification and fermentative co-production of xylitol and lipids using Rhodosporidium toruloides NCIM 3547en_UK
dc.typeArticleen_UK

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