Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

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dc.contributor.authorTothill, Alexander M.
dc.contributor.authorPartridge, Matthew
dc.contributor.authorJames, Stephen W.
dc.contributor.authorTatam, Ralph P.
dc.date.accessioned2017-02-15T15:16:37Z
dc.date.available2017-02-15T15:16:37Z
dc.date.issued2017-02-15
dc.description.abstractA 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay.en_UK
dc.identifier.citationTothill A, Partridge M, James SW, Tatam R, Fabrication and optimisation of a fused filament 3D-printed microfluidic platform, Journal of Micromechanics and Microengineering, Volume 27, Issue 3, article number 035018en_UK
dc.identifier.issn0960-1317
dc.identifier.urihttp://dx.doi.org/10.1088/1361-6439/aa5ae3
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/11449
dc.language.isoenen_UK
dc.publisherIOP Publishing: Hybrid Open Accessen_UK
dc.rightsAttribution 3.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subject3D-printingen_UK
dc.subjectmicrofluidicsen_UK
dc.subjectdevicesen_UK
dc.subjectglucoseen_UK
dc.subjectenzymaticen_UK
dc.titleFabrication and optimisation of a fused filament 3D-printed microfluidic platformen_UK
dc.typeArticleen_UK

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