Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis

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dc.contributor.authorLu, Minrui
dc.contributor.authorSha, Yuanyuan
dc.contributor.authorKumar, Vinod
dc.contributor.authorXu, Zhaoxian
dc.contributor.authorZhai, Rui
dc.contributor.authorJin, Mingjie
dc.date.accessioned2024-03-21T12:00:29Z
dc.date.available2024-03-21T12:00:29Z
dc.date.issued2024-03-18
dc.description.abstractAs a sustainable and renewable alternative to petroleum fuels, advanced biofuels shoulder the responsibility of energy saving, emission reduction and environmental protection. Traditional engineering of cell factories for production of advanced biofuels lacks efficient high-throughput screening tools and regulating systems, impeding the improvement of cellular productivity and yield. Transcription factor-based biosensors have been widely applied to monitor and regulate microbial cell factory products due to the advantages of fast detection and in-situ screening. This review updates the design and application of transcription factor-based biosensors tailored for advanced biofuels and related intermediates. The construction and genetic parts selection principle of biosensors are discussed. Strategies to enhance the performance of biosensor, including regulating promoter strength and RBS strength, optimizing plasmid copy number, implementing genetic amplifier, and modulating the structure of transcription factor, have also been summarized. We further review the application of biosensors in high-throughput screening of new metabolic engineering targets, evolution engineering, confirmation of protein function, and dynamic regulation of metabolic flux for higher production of advanced biofuels. At last, we discuss the current limitations and future trends of transcription factor-based biosensors.en_UK
dc.identifier.citationMitchler MM, Garcia JM, Montero NE, Williams GJ. (2024) Transcription factor-based biosensors: a molecular-guided approach for natural product engineering. Biotechnology Advances, Volume 72, May–June 2024, Article number 10833en_UK
dc.identifier.issn0734-9750
dc.identifier.urihttps://doi.org/10.1016/j.biotechadv.2024.108339
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/21055
dc.language.isoen_UKen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTranscription-factor-based biosensoren_UK
dc.subjectAdvanced biofuelsen_UK
dc.subjectSynthetic biologyen_UK
dc.subjectHigh-throughput screeningen_UK
dc.subjectDynamic controlen_UK
dc.subjectAdaptive evolutionen_UK
dc.titleTranscription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesisen_UK
dc.typeArticleen_UK
dcterms.dateAccepted2024-02-18

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