High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system

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dc.contributor.authorNarisetty, Vivek
dc.contributor.authorAmraoui, Yassin
dc.contributor.authorAbdullah, Alamri
dc.contributor.authorAhmad, Ejaz
dc.contributor.authorAgrawal, Deepti
dc.contributor.authorParameswaran, Binod
dc.contributor.authorPandey, Ashok
dc.contributor.authorGoel, Saurav
dc.contributor.authorKumar, Vinod
dc.date.accessioned2021-06-29T12:20:22Z
dc.date.available2021-06-29T12:20:22Z
dc.date.issued2021-06-26
dc.description.abstractDownstream processing of chemicals obtained from fermentative route is challenging and cost-determining factor of any bioprocess. 2,3-Butanediol (BDO) is a promising chemical building block with myriad applications in the polymer, food, pharmaceuticals, and fuel sector. The current study focuses on the recovery and purification of BDO produced (68.2 g/L) from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii. Studies involving screening and optimization of aqueous-two phase system (ATPS) revealed that 30% w/v (NH4)2SO4 addition to clarified fermentation broth facilitated BDO extraction in isopropanol (0.5 v/v), with maximum recovery and partition coefficient being 97.9 ± 4.6% and 45.5 ± 3.5, respectively. The optimized protocol was repeated with unfiltered broth containing 68.2 g/L BDO, cell biomass, and unspent protein, which led to the partitioning of 66.7 g/l BDO, 2.0 g/L xylose and 9.0 g/L acetic acid into organic phase with similar BDO recovery (97%) and partition coefficient (45).en_UK
dc.identifier.citationNarisetty V, Amraoui Y, Abdullah A, et al., (2021) High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system. Bioresource Technology, Volume 337, October 2021, Article number 125463en_UK
dc.identifier.issn0960-8524
dc.identifier.urihttps://doi.org/10.1016/j.biortech.2021.125463
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/16820
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectIsopropanolen_UK
dc.subjectAmmonium sulphateen_UK
dc.subjectAqueous two-phase systemen_UK
dc.subject2,3-Butanediolen_UK
dc.subjectEnterobacter ludwigiien_UK
dc.subjectXyloseen_UK
dc.titleHigh yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction systemen_UK
dc.typeArticleen_UK

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