High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system
dc.contributor.author | Narisetty, Vivek | |
dc.contributor.author | Amraoui, Yassin | |
dc.contributor.author | Abdullah, Alamri | |
dc.contributor.author | Ahmad, Ejaz | |
dc.contributor.author | Agrawal, Deepti | |
dc.contributor.author | Parameswaran, Binod | |
dc.contributor.author | Pandey, Ashok | |
dc.contributor.author | Goel, Saurav | |
dc.contributor.author | Kumar, Vinod | |
dc.date.accessioned | 2021-06-29T12:20:22Z | |
dc.date.available | 2021-06-29T12:20:22Z | |
dc.date.issued | 2021-06-26 | |
dc.description.abstract | Downstream processing of chemicals obtained from fermentative route is challenging and cost-determining factor of any bioprocess. 2,3-Butanediol (BDO) is a promising chemical building block with myriad applications in the polymer, food, pharmaceuticals, and fuel sector. The current study focuses on the recovery and purification of BDO produced (68.2 g/L) from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii. Studies involving screening and optimization of aqueous-two phase system (ATPS) revealed that 30% w/v (NH4)2SO4 addition to clarified fermentation broth facilitated BDO extraction in isopropanol (0.5 v/v), with maximum recovery and partition coefficient being 97.9 ± 4.6% and 45.5 ± 3.5, respectively. The optimized protocol was repeated with unfiltered broth containing 68.2 g/L BDO, cell biomass, and unspent protein, which led to the partitioning of 66.7 g/l BDO, 2.0 g/L xylose and 9.0 g/L acetic acid into organic phase with similar BDO recovery (97%) and partition coefficient (45). | en_UK |
dc.identifier.citation | Narisetty V, Amraoui Y, Abdullah A, et al., (2021) High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system. Bioresource Technology, Volume 337, October 2021, Article number 125463 | en_UK |
dc.identifier.issn | 0960-8524 | |
dc.identifier.uri | https://doi.org/10.1016/j.biortech.2021.125463 | |
dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/16820 | |
dc.language.iso | en | en_UK |
dc.publisher | Elsevier | en_UK |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Isopropanol | en_UK |
dc.subject | Ammonium sulphate | en_UK |
dc.subject | Aqueous two-phase system | en_UK |
dc.subject | 2,3-Butanediol | en_UK |
dc.subject | Enterobacter ludwigii | en_UK |
dc.subject | Xylose | en_UK |
dc.title | High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system | en_UK |
dc.type | Article | en_UK |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- High_yield_recovery_of_2,3-butanediol_fermented_broth-2021.pdf
- Size:
- 1.52 MB
- Format:
- Adobe Portable Document Format
- Description:
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.63 KB
- Format:
- Item-specific license agreed upon to submission
- Description: