Development of a database and its use in the Investigation of Interferences in SRM assay design

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dc.contributor.advisorBessant, Conrad
dc.contributor.authorDokpesi, Oshiobugie
dc.date.accessioned2015-09-10T15:27:03Z
dc.date.available2015-09-10T15:27:03Z
dc.date.issued2013-04
dc.description.abstractSelected Reaction Monitoring (SRM), is a form of mass spectrometry that guarantees high throughput and also a high level of selectivity and specificity. Performing SRM experiments requires the development of assays to aid in peptide identification. This is a time consuming and expensive process thus biological researchers have come up with bioinformatics solutions for the design of SRM assay. The accuracy of these bioinformatics methods is quite high and the next step is to optimise the process by tackling the interference issue. As various analytes may have the same signals within an SRM experiment and thus interfere with each other’s signals, different solutions are being derived to tackle the issue. This thesis describes the development of a SRM transition database to store peptide and transition data, software to populate the database and also software to retrieve the data from the database. Finally the database is tested with the MRMaid transitions for the human proteome which were mined from the PRIDE database and the results analysed to investigate the transition interference issue. The database currently contains data for 20220 proteins and approximately 870,000 tryptic peptides from the human proteome.en_UK
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/9414
dc.language.isoenen_UK
dc.publisherCranfield Universityen_UK
dc.rights© Cranfield University 2013. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright owner.en_UK
dc.subjectBioinformaticsen_UK
dc.subjectProteomicsen_UK
dc.subjectPeptidesen_UK
dc.subjectTransitionsen_UK
dc.subjectSpectrometryen_UK
dc.titleDevelopment of a database and its use in the Investigation of Interferences in SRM assay designen_UK
dc.typeThesis or dissertationen_UK
dc.type.qualificationlevelMastersen_UK
dc.type.qualificationnameMSc by Researchen_UK

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PhD, EngD and MSc by research theses (Cranfield Health)