Self-assembled microfiber-like biohydrogel for ultrasensitive whole-cell electrochemical biosensing in microdroplets

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dc.contributor.authorMa, Xiao-Meng
dc.contributor.authorWang, Jian-Wei
dc.contributor.authorZhao, Li-Ting
dc.contributor.authorZhang, Yafei
dc.contributor.authorLiu, Jun-Ying
dc.contributor.authorWang, Songmei
dc.contributor.authorZhu, Daochen
dc.contributor.authorYang, Zhugen
dc.contributor.authorYong, Yang-Chun
dc.date.accessioned2023-02-15T08:40:20Z
dc.date.available2023-02-15T08:40:20Z
dc.date.issued2023-01-27
dc.description.abstractA novel microfiber-like biohydrogel was fabricated by a facile approach relying on electroactive bacteria-induced graphene oxide reduction and confined self-assembly in a capillary tube. The microfiber-like biohydrogel (d = ∼1 mm) embedded high-density living cells and activated efficient electron exchange between cells and the conductive graphene network. Further, a miniature whole-cell electrochemical biosensing system was developed and applied for fumarate detection under −0.6 V (vs Ag/AgCl) applied potential. Taking advantage of its small size, high local cell density, and excellent electron exchange, this microfiber-like biohydrogel-based sensing system reached a linear calibration curve (R2 = 0.999) ranging from 1 nM to 10 mM. The limit of detection obtained was 0.60 nM, which was over 1300 times lower than a traditional biosensor for fumarate detection in 0.2 μL microdroplets. This work opened a new dimension for miniature whole-cell electrochemical sensing system design, which provided the possibility for bioelectrochemical detection in small volumes or three-dimensional local detection at high spatial resolutions.en_UK
dc.identifier.citationMa XM, Wang JW, Zhao LT, et al., (2023) Self-assembled microfiber-like biohydrogel for ultrasensitive whole-cell electrochemical biosensing in microdroplets, Analytical Chemistry, Volume 95, Issue 5, February 2023, pp. 2628-2632en_UK
dc.identifier.eissn1520-6882
dc.identifier.issn0003-2700
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.2c05155
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/19182
dc.language.isoenen_UK
dc.publisherAmerican Chemical Societyen_UK
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.titleSelf-assembled microfiber-like biohydrogel for ultrasensitive whole-cell electrochemical biosensing in microdropletsen_UK
dc.typeArticleen_UK

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