Analysis of MRSA Staphylococcal Chromosome cassette mecA status from next generation sequence data
Date published
2012-03
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Cranfield University
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SAS
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Thesis or dissertation
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Abstract
NGS sequencing libraries prepared on an Illumina NGS platform for 10 isolates of Staphylococcus aureus were analysed. After extensive pre-processing to address library quality issues, for each isolate the status of the Staphylococcal Chromosome Cassette, and its mecA gene specifying resistance to meticillin, was determined. All mecA-positive isolates encoded canonical mecA. None encoded the new variant mecA identified in strain LGA251.
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Github
Keywords
core genome, accessory genome, NGS sequencing libraries, drug resistance
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Biotechnology and Biological (BBSRC)