Precise and rapid solvent-assisted geometric protein self-patterning with submicron spatial resolution for scalable fabrication of microelectronic biosensors
dc.contributor.author | Tsutsumi, Jun'ya | |
dc.contributor.author | Turner, Anthony P. F. | |
dc.contributor.author | Mak, Wing Cheung | |
dc.date.accessioned | 2021-01-13T11:03:30Z | |
dc.date.available | 2021-01-13T11:03:30Z | |
dc.date.issued | 2021-01-08 | |
dc.description.abstract | We report a novel manufacturing method for microelectronic biosensors with a one-step wet/de-wet micropatterning of proteins onto printed metal microelectrode array supported by a hydrophobic perfluoropolymer substrate. The method exploits the phenomenon that protein adsorption toward a hydrophobic perfluoropolymer surface is drastically suppressed by self-assembly of proteins into micelles in a solvent-buffer mixture, followed by a rapid wet/de-wet process to localize the protein molecules onto the hydrophilic microelectrodes. The method affords protein micropatterning with 5 mm spatial resolution while preserving biological function and is applicable to various proteins such as albumin, enzymes, antibodies and avidin . By combining the protein micropatterning with metal nanoparticle chemisorption printing of the microelectrode array, we demonstrate as a model, facile fabrication of a microelectronic glucose biosensor supporting convergent analyte diffusion and thus showing typical steady-state I-V characteristics and fast steady-state current response (~20-60 s) together with an ultra-wide linear dynamic range (2-100 mM). Our findings provide a new technical solution for precise and accurate coupling of biomolecules with a microelectronic array interface with important implications for the scale up and manufacture of diagnostics, biofuel cells and bioelectronic devices. | en_UK |
dc.identifier.citation | Tsutsumi J, Turner APF, Cheung Mak W. (2021) Precise and rapid solvent-assisted geometric protein self-patterning with submicron spatial resolution for scalable fabrication of microelectronic biosensors. Biosensors and Bioelectronics, Volume 177, April 2021, Article number 112968 | en_UK |
dc.identifier.issn | 0956-5663 | |
dc.identifier.uri | https://doi.org/10.1016/j.bios.2021.112968 | |
dc.identifier.uri | https://dspace.lib.cranfield.ac.uk/handle/1826/16166 | |
dc.language.iso | en | en_UK |
dc.publisher | Elsevier | en_UK |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | biointerfaces | en_UK |
dc.subject | bioelectronics | en_UK |
dc.subject | bioelectrochemistry | en_UK |
dc.subject | microelectronics | en_UK |
dc.subject | Protein micropatterning | en_UK |
dc.title | Precise and rapid solvent-assisted geometric protein self-patterning with submicron spatial resolution for scalable fabrication of microelectronic biosensors | en_UK |
dc.type | Article | en_UK |
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