Functional evaluation and testing of a newly developed Teleost's Fish Otolith derived biocomposite coating for healthcare

Date

2019-01-14

Supervisor/s

Journal Title

Journal ISSN

Volume Title

Publisher

Nature Research (part of Springer Nature)

Department

Type

Article

ISSN

2045-2322

Format

Free to read from

Citation

Montañez ND, Carreño H, Escobar P, et al., (2020) Functional evaluation and testing of a newly developed Teleost's Fish Otolith derived biocomposite coating for healthcare. Scientific Reports, Volume 10, January 2020, Article number 258

Abstract

Polymers such as polycaprolactone (PCL) possess biodegradability, biocompatibility and affinity with other organic media that makes them suitable for biomedical applications. In this work, a novel biocomposite coating was synthesised by mixing PCL with layers of calcium phosphate (hydroxyapatite, brushite and monetite) from a biomineral called otolith extracted from Teleost fish (Plagioscion Squamosissimus) and multiwalled carbon nanotubes in different concentrations (0.5, 1.0 and 1.5 g/L). The biocomposite coating was deposited on an osteosynthesis material Ti6Al4V by spin coating and various tests such as Fourier transformation infrared spectroscopy (FTIR), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), scratch tests, MTT reduction cytotoxicity, HOS cell bioactivity (human osteosarcoma) by alkaline phosphatase (ALP) and fluorescence microscopy were performed to comprehensively evaluate the newly developed biocoating. It was found that an increase in the concentration of carbon nanotube induced microstructural phase changes of calcium phosphate (CP) leading to the formation of brushite, monetite and hydroxyapatite. While we discovered that an increase in the concentration of carbon nanotube generally improves the adhesion of the coating with the substrate, a certain threshold exists such that the best deposition surfaces were obtained as PCL/CP/CNT 0.0 g/L and PCL/CP/CNT 0.5 g/L.

Description

Software Description

Software Language

Github

Keywords

Calcium phosphate, multiwalled carbon nanotubes, fish otolith, polycaprolactone

DOI

Rights

Attribution 4.0 International

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