Development of sensors for the detection of clinically relevant substances using molecular imprinting
| dc.contributor.advisor | Piletsky, Sergey A. | |
| dc.contributor.advisor | Laitenberger, Peter | |
| dc.contributor.author | Fowler, Steven A. | |
| dc.date.accessioned | 2011-07-11T15:16:38Z | |
| dc.date.available | 2011-07-11T15:16:38Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | This thesis investigates the development of sensing devices based on molecularly imprinted polymers for the detection of clinically relevant analytes. Three analytes were considered, metronidazole, creatinine and propofol. A molecularly imprinted polymer (MIP) was computationally designed for metronidazole and tested using SPE techniques. This polymer was then grafted onto a transducer surface using an immobilised initiator. Amperometric and impedance detection of metronidazole were investigated. The capacitive detection of creatinine was reproduced from the literature (Panasyuk- Delaney et al., 2002) as this approach could be applied to other MIPs to form a universal platform for sensor development. However, the sensors produced using this methodology were difficult to reproduce and attempts to improve them were unsuccessful. A model for capacitive electrodes was developed to explain the obtained results. To address the key challenges found in the aforementioned work, a dual polymerisable monomer was used as a conductive anchor for the amperometric and impedance detection of propofol. The developed amperometric sensors demonstrated very high sensitivity (limit of detection was below 5 µM), although the electrodes lacked in selectivity. In conclusion, this thesis illustrates some of the key areas which need to be considered in the development of MIP-based devices and investigates some innovative solutions to these problems. | en_UK |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/5665 | |
| dc.language.iso | en | en_UK |
| dc.publisher | Cranfield University | en_UK |
| dc.rights | © Cranfield University, 2009. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder. | en_UK |
| dc.title | Development of sensors for the detection of clinically relevant substances using molecular imprinting | en_UK |
| dc.type | Thesis or dissertation | en_UK |
| dc.type.qualificationlevel | Doctoral | en_UK |
| dc.type.qualificationname | PhD | en_UK |