Microbiome and infectivity studies reveal complex polyspecies tree disease in Acute Ock Decline

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dc.contributor.authorDenman, Sandra
dc.contributor.authorDoonan, James
dc.contributor.authorRansom-Jones, Emma
dc.contributor.authorBroberg, Martin
dc.contributor.authorPlummer, Sarah
dc.contributor.authorKirk, Susan
dc.contributor.authorScarlett, Kelly
dc.contributor.authorGriffiths, Andrew R.
dc.contributor.authorKaczmarek, Maciej
dc.contributor.authorForster, Jack
dc.contributor.authorPeace, Andrew
dc.contributor.authorGolyshin, Peter N.
dc.contributor.authorHassard, Francis
dc.contributor.authorBrown, Nathan
dc.contributor.authorKenny, John G.
dc.contributor.authorMcDonald, James E.
dc.date.accessioned2017-11-16T16:28:07Z
dc.date.available2017-12-13T16:28:07Z
dc.date.issued2017-10-13
dc.description.abstractDecline-diseases are complex and becoming increasingly problematic to tree health globally. Acute Oak Decline (AOD) is characterized by necrotic stem lesions and galleries of the bark-boring beetle, Agrilus biguttatus, and represents a serious threat to oak. Although multiple novel bacterial species and Agrilus galleries are associated with AOD lesions, the causative agent(s) are unknown. The AOD pathosystem therefore provides an ideal model for a systems-based research approach to address our hypothesis that AOD lesions are caused by a polymicrobial complex. Here we show that three bacterial species, Brenneria goodwinii, Gibbsiella quercinecans and Rahnella victoriana, are consistently abundant in the lesion microbiome and possess virulence genes used by canonical phytopathogens that are expressed in AOD lesions. Individual and polyspecies inoculations on oak logs and trees demonstrated that B. goodwinii and G. quercinecans cause tissue necrosis and, in combination with A. biguttatus, produce the diagnostic symptoms of AOD. We have proved a polybacterial cause of AOD lesions, providing new insights into polymicrobial interactions and tree disease. This work presents a novel conceptual and methodological template for adapting Koch’s postulates to address the role of microbial communities in disease.en_UK
dc.identifier.citationDenman S, Doonan J, Ransom-Jones E, et al., (2018) Microbiome and infectivity studies reveal complex polyspecies tree disease in Acute Oak Decline. The ISME Journal, Volume 12, February 2018, pp. 386-399en_UK
dc.identifier.cris18725182
dc.identifier.issn1751-7362
dc.identifier.urihttp://dx.doi.org/10.1038/ismej.2017.170
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/12750
dc.language.isoenen_UK
dc.publisherNature Publishing Groupen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEnvironmental microbiologyen_UK
dc.subjectForest ecologyen_UK
dc.subjectInfectious-disease diagnosticsen_UK
dc.subjectMicrobiomeen_UK
dc.subjectMolecular ecologyen_UK
dc.titleMicrobiome and infectivity studies reveal complex polyspecies tree disease in Acute Ock Declineen_UK
dc.typeArticleen_UK

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