Complex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperature

Simple item page
dc.contributor.authorSchmidt-Heydt, Markus
dc.contributor.authorAbdel-Hadi, Ahmed
dc.contributor.authorMagan, Naresh
dc.contributor.authorGeisen, Rolf
dc.date.accessioned2010-01-06T12:49:03Z
dc.date.available2010-01-06T12:49:03Z
dc.date.issued2009-11-15
dc.description.abstractA microarray analysis was performed to study the effect of varying combinations of water activity and temperature on the activation of aflatoxin biosynthesis genes in Aspergillus flavus grown on YES medium. Generally A. flavus showed expression of the aflatoxin biosynthetic genes at all parameter combinations tested. Certain combinations of aw and temperature, especially combinations which imposed stress on the fungus resulted in a significant reduction of the growth rate. At these conditions induction of the whole aflatoxin biosynthesis gene cluster occurred, however the produced aflatoxin B1 was low. At all other combinations (25 °C/0.95 and 0.99; 30 °C/0.95 and 0.99; 35 °C/0.95 and 0.99) a reduced basal level of cluster gene expression occurred. At these combinations a high growth rate was obtained as well as high aflatoxin production. When single genes were compared, two groups with different expression profiles in relation to water activity/temperature combinations occurred. These two groups were co-ordinately localized within the aflatoxin gene cluster. The ratio of aflR/aflJ expression was correlated with increased aflatoxin biosynthesis.en_UK
dc.identifier.citationMarkus Schmidt-Heydt, Ahmed Abdel-Hadi, Naresh Magan, Rolf Geisen, Complex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperature, International Journal of Food Microbiology, Volume 135, Issue 3, 15 November 2009, Pages 231-237en_UK
dc.identifier.issn0168-1605
dc.identifier.urihttp://dx.doi.org/10.1016/j.ijfoodmicro.2009.07.026
dc.identifier.urihttp://hdl.handle.net/1826/4103
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.subjectAflatoxinen_UK
dc.subjectMicroarrayen_UK
dc.subjectWater activityen_UK
dc.subjectTemperatureen_UK
dc.subjectGene expressionen_UK
dc.titleComplex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperatureen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
aflatoxin_biosynthesis_gene_cluster_of_Aspergillus_flavus-2009.pdf
Size:
309.46 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.88 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications - Cranfield Health