Enzymes targeting distinct hydrolysis blind-spots of thermal and biological pre-treatments significantly uplift biogas production

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dc.contributor.authorNasar, Nasreen
dc.contributor.authorPizzagalli, Giulia
dc.contributor.authorCoulon, Frederic
dc.contributor.authorBajón Fernández, Yadira
dc.date.accessioned2025-03-12T12:38:58Z
dc.date.available2025-03-12T12:38:58Z
dc.date.freetoread2025-03-12
dc.date.issued2025-06
dc.date.pubOnline2025-03-10
dc.description.abstractThermal hydrolysis process (THP) and biological hydrolysis (BH) are key pre-treatment technologies for anaerobic digestion (AD), termed advanced anaerobic digesters (AADs). They target the rate-limiting hydrolysis step in AD. This study evaluates full-scale pre-treatments for macromolecule bias and the implementation of hydrolysis enzymes to enhance biogas yield. Findings show THP significantly improves protein and carbohydrate solubilisation by 30% and 25%, respectively, but fully hydrolyses only carbohydrates. In contrast, BH targets fibres and proteins, achieving 35% and 23% solubilisation, and only partially hydrolyses carbohydrates. Biomethane potential (BMP) tests indicate that protease enzymes raise biomethane yield by 20-30% for AAD with THP pre-treatment. In comparison, α-amylase increases it by over 30% for AAD with BH pre-treatment. This study tailors enzyme selection and dosage to specifically address the unique "hydrolysis blind spot" of each pre-treatment, providing a strategic framework to enhance AD technologies by an improved understanding of macromolecule selectivity and their transformation pathways.
dc.description.journalNameBioresource Technology
dc.format.mediumPrint-Electronic
dc.identifier.citationNasar N, Pizzagalli G, Coulon F, Bajón Fernández Y. (2025) Enzymes targeting distinct hydrolysis blind-spots of thermal and biological pre-treatments significantly uplift biogas production. Bioresource Technology, Volume 426, June 2025, Article number 132353en_UK
dc.identifier.eissn1873-2976
dc.identifier.elementsID566195
dc.identifier.issn0960-8524
dc.identifier.paperNo132353
dc.identifier.urihttps://doi.org/10.1016/j.biortech.2025.132353
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23596
dc.identifier.volumeNo426
dc.languageEnglish
dc.language.isoen
dc.publisherElsevieren_UK
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0960852425003190?via%3Dihub
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject3107 Microbiologyen_UK
dc.subject31 Biological Sciencesen_UK
dc.subject3106 Industrial Biotechnologyen_UK
dc.subjectAdvanced anaerobic digestionen_UK
dc.subjectBiomethane yielden_UK
dc.subjectMacromolecule selectivityen_UK
dc.subjectTailored enzyme dosingen_UK
dc.subjectBiotechnologyen_UK
dc.subject3001 Agricultural biotechnologyen_UK
dc.titleEnzymes targeting distinct hydrolysis blind-spots of thermal and biological pre-treatments significantly uplift biogas productionen_UK
dc.typeArticle
dc.type.subtypeJournal Article
dcterms.dateAccepted2025-03-04

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