Bioprocessing of fermentable sugars derived from water hyacinth into microbial lipids and single cell proteins by oleaginous yeast Rhodosporidium toruloides NCIM 3547

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dc.contributor.authorAlankar, Senthilnathan Sri Laxma
dc.contributor.authorSajesh, Nithianandam
dc.contributor.authorRastogi, Shrestha
dc.contributor.authorSakhuja, Simar
dc.contributor.authorRajeswari, Gunasekaran
dc.contributor.authorKumar, Vinod
dc.contributor.authorChandel, Anuj Kumar
dc.contributor.authorJacob, Samuel
dc.date.accessioned2021-10-25T13:06:48Z
dc.date.available2021-10-25T13:06:48Z
dc.date.issued2021-10-09
dc.description.abstractIn this study, we employed microwave-acid pretreatment for water hyacinth (WH) to obtain liquid hydrolysate that contains sugars derived from holocellulosic components of biomass for further oleaginous yeast fermentation. In order to remove the inhibitors such as furans after acid treatment, detoxification of hydrolysate was done and we compared the efficiency of this step with non-detoxified hydrolysate towards capability of the Rhodosporidium toruloides NCIM 3547 (an oleaginous yeast) to produce microbial lipid and single cell protein. The results indicated that the reducing sugar concentration was found to be higher in non-detoxified hydrolysate (65.41 g/L) than detoxified one (59.18 g/L). When the non-detoxified liquid hydrolysate was supplemented with yeast extract as a complex organic source for R. toruloides, resulted in a maximum lipid yield of about 0.813 ± 0.041 (g/g) and 53.60 ± 2.68 (g/g) of single cell protein content with 0.038 g/L/d of protein productivity. Two kinetic models, hybrid Logistic-Monod and Luedeking-Piret, were employed to assess the microbial growth and the substrate utilization that were found to be in well agreement with the experimental data with a coefficient of determination (R2) value ranging from 0.95 to 0.99 thereby demonstrating the efficiency of the hydrolysate supplemented media. Furthermore, GC-MS analysis of transesterified lipids revealed the presence of various FAME (fatty acid methyl esters) and also the presence of increased levels of total saturated fatty acids (35.03%) advocates its high potential in biodiesel production. This study demonstrates the feasibility of sustainable valorization of WH-derived liquid hydrolysate towards a greener biorefinery framework.en_UK
dc.identifier.citationAlankar SS, Sajesh N, Rastogi S, et al., (2023) Bioprocessing of fermentable sugars derived from water hyacinth into microbial lipids and single cell proteins by oleaginous yeast Rhodosporidium toruloides NCIM 3547. Biomass Conversion and Biorefinery, Volume 13, November 2023, pp. 15435-15449en_UK
dc.identifier.issn2190-6815
dc.identifier.urihttps://doi.org/10.1007/s13399-021-02007-6
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/17198
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectWater hyacinthen_UK
dc.subjectLiquid hydrolysateen_UK
dc.subjectWaste valorizationen_UK
dc.subjectOleaginous yeasten_UK
dc.subjectMicrobial lipidsen_UK
dc.subjectSingle cell proteinen_UK
dc.titleBioprocessing of fermentable sugars derived from water hyacinth into microbial lipids and single cell proteins by oleaginous yeast Rhodosporidium toruloides NCIM 3547en_UK
dc.typeArticleen_UK

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