Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease

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dc.contributor.authorJia, Wenjing-
dc.contributor.authorWhitehead, Rebekah N.-
dc.contributor.authorGriffiths, Lesley-
dc.contributor.authorDawson, Claire-
dc.contributor.authorBai, Hao-
dc.contributor.authorWaring, Rosemary H.-
dc.contributor.authorRamsden, David B.-
dc.contributor.authorHunter, John O.-
dc.contributor.authorCauchi, Michael-
dc.contributor.authorBessant, Conrad M.-
dc.contributor.authorFowler, Dawn P.-
dc.contributor.authorWalton, Christopher-
dc.contributor.authorTurner, Claire-
dc.contributor.authorCole, Jeffrey A.-
dc.date.accessioned2013-01-23T23:01:39Z
dc.date.available2013-01-23T23:01:39Z
dc.date.issued2012-06-01T00:00:00Z-
dc.description.abstractThe relative abundance of different groups of sulphate-reducing bacteria (SRB) in faecal DNA collected before and after therapy from patients suffering from Crohn's disease (CD), irritable bowel syndrome (IBS) or ulcerative colitis (UC) has been compared with that from healthy controls. Growth tests revealed that SRB were not more abundant in samples from patients with CD before treatment than in the healthy control group. For most of the 128 samples available, these preliminary results were confirmed using degenerate PCR primers that amplify the dsrAB gene. However, some samples from patients with CD before treatment contained a growth inhibitor that was absent from IBS or UC samples. In-depth sequencing of PCR-generated dsrB fragments revealed that the diversity detected was surprisingly low, with only eight strains of SRB and the sulphite-reducing bacterium, Bilophila wadsworthia, detected above the 0.1% threshold. The proportion of the two major species detected, B.wadsworthia and Desulfovibrio piger, was as high as 93.5% of the total SRB population in the healthy control group and lower in all patient groups. Four previously undescribed species were found: it is impossible to predict whether they are sulphate or sulphite-reducing bacteria.en_UK
dc.identifier.citationWenjing Jia, Rebekah N. Whitehead, Lesley Griffiths, Claire Dawson, Hao Bai, Rosemary H. Waring, David B. Ramsden, John O. Hunter, Michael Cauchi, Conrad Bessant, Dawn P. Fowler, Christopher Walton, Claire Turner and Jeffrey A. Cole. Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease. FEMS Immunology and Medical Microbiology, Volume 65, Issue 1, pages 55–68, June 2012.
dc.identifier.issn0928-8244-
dc.identifier.urihttp://dx.doi.org/10.1111/j.1574-695X.2012.00935.x-
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/7779
dc.rightsThe definitive version is available at www3.interscience.wiley.com
dc.titleDiversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel diseaseen_UK
dc.typeArticle-

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