Citation:
E. V. Piletska, S. A. Piletsky, S. Subrahmanyam, K. Karim and A. P. F. Turner, A new reactive polymer suitable for covalent immobilisation and monitoring of primary amines, Polymer, Volume 42, Issue 8 , April 2001, Pages 3603-3608
Abstract:
A new polymer able to react with primary amines was synthesised from allyl
mercaptan, o-phtalic dialdehyde and ethylene glycol dimethacrylate by radical
polymerisation. Reactive thioacetale formed by allyl mercaptan and dialdehyde
can bind primary amino groups without additional pre-activation forming
fluorescent isoindole complex. It gives a great opportunity to monitor binding
and loading of the amino compounds on the reactive surface. The reactive polymer
is found to be an effective matrix for immobilisation of the proteins and other
amino containing compounds in affinity chromatography and could be used for
their detection in solution.