Citation:
Sergey A. Piletsky, Elena V. Piletska, Alessandra Bossi, Khalku Karim, Philip
Lowe and Anthony P. F. Turner, Substitution of antibodies and receptors with
molecularly imprinted polymers in enzyme-linked and fluorescent assays,
Biosensors and Bioelectronics, Volume 16, Issues 9-12, December 2001, Pages 701-
707
Abstract:
A new technique for coating microtitre plates with molecularly imprinted
polymers (MIP), specific for low-molecular weight analytes (epinephrine,
atrazine) and proteins is presented. Oxidative polymerization was performed in
the presence of template; monomers: 3-aminophenylboronic acid, 3-
thiopheneboronic acid and aniline were polymerized in water and the polymers
were grafted onto the polystyrene surface of the microplates. It was found that
this process results in the creation of synthetic materials with antibody-like
binding properties. It was shown that the MIP-coated microplates are
particularly useful for assay development. The high stability of the polymers
and good reproducibility of the measurements make MIP coating an attractive
alternative to conventional antibodies or receptors used in ELISA.
