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Please use this identifier to cite or link to this item: http://dspace.lib.cranfield.ac.uk/handle/1826/802

Document Type: Article
Title: Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays
Authors: Piletsky, Sergey A.
Piletska, Elena V.
Bossi, Alessandra
Karim, K.
Lowe, P.
Turner, Anthony P. F.
Issue Date: 2001
Citation: Sergey A. Piletsky, Elena V. Piletska, Alessandra Bossi, Khalku Karim, Philip Lowe and Anthony P. F. Turner, Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays, Biosensors and Bioelectronics, Volume 16, Issues 9-12, December 2001, Pages 701- 707
Abstract: A new technique for coating microtitre plates with molecularly imprinted polymers (MIP), specific for low-molecular weight analytes (epinephrine, atrazine) and proteins is presented. Oxidative polymerization was performed in the presence of template; monomers: 3-aminophenylboronic acid, 3- thiopheneboronic acid and aniline were polymerized in water and the polymers were grafted onto the polystyrene surface of the microplates. It was found that this process results in the creation of synthetic materials with antibody-like binding properties. It was shown that the MIP-coated microplates are particularly useful for assay development. The high stability of the polymers and good reproducibility of the measurements make MIP coating an attractive alternative to conventional antibodies or receptors used in ELISA.
URI: http://dx.doi.org/10.1016/S0956-5663(01)00234-2
Appears in Collections:Staff publications - Cranfield University at Silsoe

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