Citation:
Sergey Piletsky, Elena Piletska, Kal Karim, Graham Foster, Colton Legge and
Anthony Turner, Custom synthesis of molecular imprinted polymers for
biotechnological application: preparation of a polymer selective for tylosin,
Analytica Chimica Acta, Volume 504, Issue 1, 16 February 2004, Pages 123-130
Abstract:
A molecularly imprinted polymer (MIP) selective for tylosin was designed and
synthesised using a computational method (MIP “dialling”). In re-binding
experiments the MIP demonstrated high affinity for tylosin in aqueous solutions
and in organic solvents. The synthesised polymer was tested for re-binding with
the template and related metabolites such as tylactone, narbomycin and
picromycin. The HPLC analysis showed that the computationally designed polymer
is specific and capable of separating the template from its structural
analogues. The MIP was capable of recovering tylosin from broth samples. The
polymer capacity for tylosin was estimated as 6.4 mg/g for MIP, which was
suitable for practical application and tylosin recovery from broth samples.
Among the advantages of this was the possibility to adsorb tylosin from a
complex media with easy removal of oils and other impurities which are present
in significant quantities, which can create problems for its chromatographic
purification procedure. The MIP “dialling” procedure can have a general
significance for the fast preparation of specific adsorbents for
biotechnological appli
