Citation:
Duane Carey, Gavin Rhys Lloyd, Neil Shepherd, Nick Stone, Catherine Kendall and Toby Breckon.
Correlating histology and spectroscopy to differentiate pathologies of the colon. Proceedings of the 16th Annual Conference Medical Image Understanding and Analysis (MIUA 2012). Swansea, 9-11 July 2012, pages 243-248
Abstract:
The techniques and procedures associated with histology are, in most cases,
suitable for the diagnosis of colonic carcinomas. However, in cases such as
epithelial misplacement the morphology of a stained tissue sample is homologous
to that of cancer. This can lead to patients being misdiagnosed and undergoing
unnecessary surgery. To prevent this surgery we suggest that the epithelium of
tissue samples be examined using infrared (IR) spectroscopy. In this study, IR
maps of tissue sections were registered to standard histology images so that
epithelial specific spectra could be collected. The differences between these
spectra were explored by using Principal Component Analysis (PCA). This paper
provides a novel protocol detailing how histology specific spectra can be
collected. The potential usefulness of these spectra is demonstrated through the
separation of epithelial misplacement cases and colonic carcinomas within PCA
space.
