Citation:
M. Salih, I. Mytilinaios, H.K. Schofield and R.J.W. Lambert. Modelling of bacterial growth with shifts in temperature using automated methods
with Listeria monocytogenes and Pseudomonas aeruginosa as examples. International Journal of Food Microbiology, Volume 155, Issues 1–2, 2 April 2012, Pages 29-35.
Abstract:
Time to detection (TTD) measurements using turbidometry allow a straightforward
method for the measurement of bacterial growth rates under isothermal
conditions. Growth rate measurements were carried out for Listeria monocytogenes
at 25, 30 and 37°C and for Pseudomonas aeruginosa over the temperature range 25
to 45°C. The classical three-parameter logistic model was rearranged to provide
the theoretical foundation for the observed TTD. A model was subsequently
developed for the analysis of TTD data from non-isothermal studies based on the
Malthusian approximation of the logistic model. The model was able to predict
the TTD for cultures of L. monocytogenes or P. aeruginosa undergoing simple
temperature shunts (e.g. 25 to 37°C and vice versa), and for a multiple
temperature shunt for L. monocytogenes (25-37-25-37°C and 37-25-37-25°C) over a
period of 24h. In no case did a temperature shunt induce a
