dc.contributor.author |
Altintas, Zeynep |
- |
dc.contributor.author |
Uludag, Yildiz |
- |
dc.contributor.author |
Gurbuz, Yasar |
- |
dc.contributor.author |
Tothill, Ibtisam E. |
- |
dc.date.accessioned |
2012-04-18T23:02:27Z |
|
dc.date.available |
2012-04-18T23:02:27Z |
|
dc.date.issued |
2012-01-27T00:00:00Z |
- |
dc.identifier.citation |
Zeynep Altintas, Yıldız Uludag, Yasar Gurbuz, Ibtisam Tothill. Development of surface chemistry for surface plasmon resonance based sensors for
the detection of proteins and DNA molecules. Analytica Chimica Acta 712 (2012) 138–144 |
|
dc.identifier.issn |
0003-2670 |
- |
dc.identifier.uri |
http://dx.doi.org/10.1016/j.aca.2011.11.026 |
- |
dc.identifier.uri |
http://dspace.lib.cranfield.ac.uk/handle/1826/7102 |
|
dc.description.abstract |
The immobilisation of biological recognition elements onto a sensor chip surface
is a crucial step for the construction of biosensors. While some of the optical
biosensors utilise silicon dioxide as the sensor surface, most of the biosensor
surfaces are coated with metals for transduction of the signal. Biological
recognition elements such as proteins can be adsorbed spontaneously on metal or
silicon dioxide substrates but this may denature the molecule and can result in
either activity reduction or loss. Self assembled monolayers (SAMs) provide an
effective method to protect the biological recognition elements from the sensor
surface, thereby providing ligand immobilisation that enables the repeated
binding and regeneration cycles to be performed without losing the immobilised
ligand, as well as additionally helping to minimise non-specific adsorption.
Therefore, in this study different surface chemistries were constructed on SPR
sensor chips to investigate protein and DNA immobilisation on Au surfaces. A
cysteamine surface and 1%, 10% and 100% mercaptoundecanoic acid (MUDA) coatings
with or without dendrimer modification were utilised to construct the various
sensor surfaces used in this investigation. A higher response was obtained for
NeutrAvidin immobilisation on dendrimer modified surfaces compared to MUDA and
cysteamine layers, however, protein or DNA capture responses on the immobilised
NeutrAvidin did not show a similar higher response when dendrimer modified
surfaces were used. |
en_UK |
dc.publisher |
Elsevier Science B.V., Amsterdam. |
en_UK |
dc.title |
Development of surface chemistry for surface plasmon resonance based sensors for
the detection of proteins and DNA molecules |
en_UK |
dc.type |
Article |
- |