Citation:
Elena Piletska, Sergey Piletsky, Kal Karim, Ewald Terpetschnig and Anthony
Turner, Biotin –specific synthetic receptors prepared using molecular
imprinting, Analytica Chimica Acta, Volume 504, Issue 1, 16 February 2004, Pages
179-1
Abstract:
The composition of new molecularly imprinted polymers (MIPs) specific for biotin
was optimised using molecular modelling software. Three functional monomers:
methacrylic acid (MAA), 2-(trifluoromethyl)acrylic acid (TFAA) and 2-acrylamido-
2-methyl-propanesulfonic acid (AMPSA), which demonstrated the highest binding
scores with biotin, were tested on their ability to generate specific binding
sites. The imprinted polymers were photografted to the surface of polystyrene
microspheres in water. The affinity of the synthetic "receptor" sites was
evaluated in binding experiments using horseradish peroxidase-labelled biotin. A
good correlation was found between the modelling results and the performance of
the materials in the template rebinding study. The dissociation constants for
all MIPs were 1.4-16.8 nM, which is sufficient for most analytical applications
where biotin is used as a label.
