Citation:
Jiong Zhang, Yanjian Wan, Yuanyuan Li, Qiongfang Zhang, Shunqing Xu, Huijun Zhu and Baihua Shu. A rapid and high-throughput quantum dots bioassay for monitoring of
perfluorooctane sulfonate in environmental water samples. Environmental Pollution, Volume 159, Issue 5, May 2011, Pages 1348-1353
Abstract:
Currently HPLC/MS is the state of the art tool for environmental/drinking water
perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal
proliferator-activated receptor-alpha (PPARα), which forms heterodimers with
retinoid X receptors (RXRs) and binds to PPAR response elements. In this
bioassay free PFOS in water samples competes with immobilized PFOS in ELISA
plates for a given amount of PPARα-RXRα. It can be determined indirectly by
immobilizing PPARα-RXRα-PFOS complex to another plate coated with PPARα antibody
and subsequent measuring the level of PPARα-RXRα by using biotin-modified PPARα-
RXRα probes-quantum dots-streptavidin detection system. The rapid and high-
throughput bioassay demonstrated a detection limit of 2.5ngL-1 with linear range
between 2.5ngL-1 and 75ngL-1. Detection results of environmental water samples
were highly consistent between the bioassay an
