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|Document Type: ||Article|
|Title: ||The stabilisation of receptor structure in low cross-linked MIPs by an
|Authors: ||Garcinuno, R. M.|
Piletska, Elena V.
Whitcombe, M. J.
Piletsky, Sergey A.
|Issue Date: ||2009|
|Citation: ||Rosa M. Garcinuno, Iva Chianella, Antonio Guerreiro, Irene Mijangos, Elena V.
Piletska, Michael J. Whitcombe and Sergey A. Piletsky, The stabilisation of
receptor structure in low cross-linked MIPs by an immobilised template, Soft
Matter, 2009, Issue 5, Pages 311–3|
|Abstract: ||In molecularly imprinted polymers (MIPs) a high level of cross-linking is
usually important for preserving the receptor structure. We propose here an
alternative approach for stabilising binding sites, which involves the use of an
immobilised template. The idea is based on the assumption that an immobilised
template will ‘‘hold’’ polymeric chains and complementary functionalities
together, preventing the collapsing of the binding sites. To test this
postulate, a range of polymers was prepared using polymerisable (2,4-diamino-6-
(methacryloyloxy)ethyl-1,3,5-triazine) and non-polymerisable (or extractable)
(2,4-diamino-6-methyl-1,3,5-triazine) templates, methacrylic acid as functional
monomer and ethylene glycol dimethacrylate as cross-linker. The level of cross-
linking was varied from 12 to 80%. Polymerisations were performed in
acetonitrile using UV initiation. Binding properties of the synthesised
materials were characterised both by HPLC and equilibrium batch binding
experiments followed by HPLC-MS or UV-visible detection. The adsorption
isotherms of polymers were obtained and fitted to the Langmuir model to
calculate dissociation constant, Kd, and concentration of binding sites for each
material. The results strongly indicate that the presence of an immobilised
template improves the affinity of MIPs containing low percentages of cross-
linker. The low cross-linked MIPs synthesised with a polymerisable template also
retain a reasonable degree of selectivity. Low crosslinked MIPs with such
binding characteristics would be useful for the creation of new types of optical
and electrochemical sensors, where induced fit or the ‘‘gate effect’’ could be
used more effectively for generating and enhancing|
|Appears in Collections:||Staff publications - Cranfield Health|
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