Citation:
Antonio R. Guerreiro, Iva Chianella, Elena Piletska, Michael J. Whitcombe,
Sergey A. Piletsky, Selection of imprinted nanoparticles by affinity
chromatography, Biosensors and Bioelectronics, Volume 24, Issue 8, 15 April
2009, Pages 2740-2743
Abstract:
Soluble molecularly imprinted nanoparticles were synthesised via iniferter
initiated polymerisation and separated by size via gel permeation
chromatography. Subsequent fractionation of these particles by affinity
chromatography allowed the separation of high affinity fractions from the
mixture of nanoparticles. Fractions selected this way possess affinity similar
to that of natural antibodies (Kd 6.6 × 10−8) M and were also able to
discriminate between related functional analogues of the templa
