Citation:
Angel Medina, Francisco M. Valle-Algarra, Misericordia Jimenez, Naresh Magan; Different sample treatment approaches for the analysis of T-2 and HT-2 toxins
from oats-based media, Journal of Chromatography B, Volume 878, Issue 23, 1 August 2010, Pages 2145-2149
Abstract:
A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in
cultures of Fusarium langsethiae in oat-based and other in vitro media. Test
media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin
stock solutions were added. Different mixtures of extraction solvent
(acetonitrile:water and methanol water), extraction times (30', 60' or 90') and
drying methods were investigated. Results showed that extraction with methanol:
water (80:20, v/v) for 90 min, drying with N-2 and subsequent analysis by LC-DAD
was the fastest and most user friendly method for detecting HT-2 and T-2 toxins
production by F. langsethiae strains grown on oat-based media at levels of 0.459
and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to
investigate toxin production of 6 F. langsethiae strains from northern Europe
and provided clear chromatograms with no interfering peaks in media with and
without glycerol as water activity modifier. (C) 2010 Elsevier B.V. All rights
reserved.
