Ascorbate enhances iron uptake into intestinal cells through formation of a
FeCl3-ascorbate complex

Thumser, Alfred E.; Rashed, Aswir Abd; Sharp, Paul A.; Lodge, John K.

dc.contributor.author	Thumser, Alfred E.	-
dc.contributor.author	Rashed, Aswir Abd	-
dc.contributor.author	Sharp, Paul A.	-
dc.contributor.author	Lodge, John K.	-
dc.date.accessioned	2011-01-17T23:01:21Z
dc.date.available	2011-01-17T23:01:21Z
dc.date.issued	2010-11-15T00:00:00Z	-
dc.identifier.citation	Alfred E. Thumser, Aswir Abd Rashed, Paul A. Sharp, John K. Lodge; Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex, Food Chemistry, Volume 123, Issue 2, 15 November 2010, Pages 281-285
dc.identifier.issn	0308-8146	-
dc.identifier.uri	http://dx.doi.org/10.1016/j.foodchem.2010.04.031	-
dc.identifier.uri	http://dspace.lib.cranfield.ac.uk/handle/1826/4738
dc.description.abstract	It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration- dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+-ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+-ascorbate complex. (C) 2010 Elsevier Ltd. All rights reserved.	en_UK
dc.language.iso	en_UK	en_UK
dc.publisher	Elsevier Science B.V., Amsterdam.	en_UK
dc.subject	Enterocyte iron uptake Ascorbate Ferric iron caco-2 cells nonheme iron colorimetric assay epithelial-cells expression transport absorption dmt1 bioavailability humans	en_UK
dc.title	Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex	en_UK
dc.type	Article	en_UK