Abstract:
A lipase-producing micro-organism was found to be a strain
of Pseudomonas aeruginosa. When it was grown batchwise in
a stirred 2 dM3 fermenter in a simple defined minimal
salts medium containing yeast extract and glucose, it
produced lipase at a level of 0.173 LU/cm3 without the
need for a lipid substrate. Batch cultures of cells were
fed glucose at constant rates. An optimum rate was found
to be 0.50 g/dm3/h and this produced a maximum lipase
activity of 0.80 LU/cm3.
Medium composition was changed until it was possible to
produce 25 g/dm3 dry weight of cells. Evidence of linear
growth due to nutient, limitations was obtained. A microcomputer
controlled protocol was used successfully to feed
glucose solutions at exponentially increasing rates.
Problems were encountered with the production of lipase
from the interaction with pyocyanin production and from
possible trace nutrient limitations.