Citation:
Francesca Bonini, Sergey Piletsky, Anthony P.F. Turner, Adolfo Speghini, Alessandra Bossi, Surface imprinted beads for the recognition of human serum albumin, Biosensors and Bioelectronics, Volume 22, Issues 9-10, Selected Papers from the Ninth World Congress On Biosensors. Toronto, Canada 10 - 12 May 2006, Alice X. J . Tang, 15 April 2007, Pages 2322-2328
Abstract:
The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the
recognition of the protein human serum albumin (HSA) is reported. In order to
create homogeneous recognition sites, covalent immobilisation of the template
HSA was exploited. The resulting imprinted beads were selective for HSA. The
indirect imprinting factor (IF) calculated from supernatant was 1.6 and the
direct IF, evaluated from the protein recovered from the beads, was 1.9. The
binding capacity was 1.4 mg/g, which is comparable to commercially available
affinity materials. The specificity of the HSA recognition was evaluated with
competitive experiments, indicating a molar ratio 4.5/1 of competitor was
necessary to displace half of the bound HSA. The recognition and binding of the
imprinted beads was also tested with a complex sample, human serum and targeted
removal of HSA without a loss of the other protein components was demonstrated.
The easy preparation protocol of derivatised beads and a good protein
recognition properties make the approach an attractive solution to analytical
and bio-analytical problems in the field of biotechnology.
