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Please use this identifier to cite or link to this item: http://dspace.lib.cranfield.ac.uk/handle/1826/3431

Document Type: Article
Title: Surface imprinted beads for the recognition of human serum albumin
Authors: Bonini, Francesca
Piletsky, Sergey A.
Turner, Anthony P. F.
Speghini, Adolfo
Bossi, Alessandra
Issue Date: 2007
Citation: Francesca Bonini, Sergey Piletsky, Anthony P.F. Turner, Adolfo Speghini, Alessandra Bossi, Surface imprinted beads for the recognition of human serum albumin, Biosensors and Bioelectronics, Volume 22, Issues 9-10, Selected Papers from the Ninth World Congress On Biosensors. Toronto, Canada 10 - 12 May 2006, Alice X. J . Tang, 15 April 2007, Pages 2322-2328
Abstract: The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology.
URI: http://dx.doi.org/10.1016/j.bios.2006.12.034
http://dspace.lib.cranfield.ac.uk/handle/1826/3431
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