Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications.

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dc.contributor.author Barton, Andrew C. -
dc.contributor.author Collyer, Stuart D. -
dc.contributor.author Davis, Frank -
dc.contributor.author Garifallou, Goulielmos-Zois -
dc.contributor.author Tsekenis, Georgios -
dc.contributor.author Tully, Elizabeth -
dc.contributor.author O'Kennedy, Richard -
dc.contributor.author Gibson, Tim -
dc.contributor.author Millner, Paul A. -
dc.contributor.author Higson, Seamus P. J. -
dc.date.accessioned 2011-10-11T07:32:55Z
dc.date.available 2011-10-11T07:32:55Z
dc.date.issued 2009-01-01T00:00:00Z -
dc.identifier.citation Andrew C. Barton, Stuart D. Collyer, Frank Davis, Goulielmos-Zois Garifallou, Georgios Tsekenis, Elizabeth Tully, Richard O'Kennedy, Tim Gibson, Paul A. Millner, Seamus P.J. Higson, Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications, Biosensors and Bioelectronics, Volume 24, Issue 5, Selected Papers from the Tenth World Congress on Biosensors Shangai, China, May 14-16, 2008, 1 January 2009, Pages 1090-1095 -
dc.identifier.issn 0956-5663 -
dc.identifier.uri http://dx.doi.org/10.1016/j.bios.2008.06.001 -
dc.identifier.uri http://dspace.lib.cranfield.ac.uk/handle/1826/3196
dc.description.abstract This paper describes the development and characterisation of labeless immunosensors for (a) the cardiac drug digoxin and (b) bovine serum albumin (BSA). Commercial screen-printed carbon electrodes were used as the basis for the sensors. Two methods were used to immobilise antibodies at the electrode surface. Aniline was electropolymerised onto these electrodes to form a thin planar film of conductive polyaniline; the polyaniline film was then utilised as a substrate to immobilise biotinylated anti-digoxin using a classical avidin- biotin affinity approach. As an alternative approach, poly(1,2-diaminobenzene) was electrodeposited onto the carbon electrodes and this modified surface was then sonochemically ablated to form an array of micropores. A second electropolymerisation step was then used to co-deposit conductive polyaniline along with antibodies for BSA within these pores to produce a microarray of polyaniline protrusions with diameters of several μm, containing entrapped anti- BSA en_UK
dc.language.iso en_UK -
dc.publisher Elsevier Science B.V., Amsterdam. en_UK
dc.subject AC impedance en_UK
dc.subject Immunosensor en_UK
dc.subject Polyaniline en_UK
dc.subject Microelectrode en_UK
dc.subject BSA en_UK
dc.subject Digoxin en_UK
dc.title Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications. en_UK
dc.type Article -


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