Citation:
Andrew C. Barton, Stuart D. Collyer, Frank Davis, Goulielmos-Zois Garifallou, Georgios Tsekenis, Elizabeth Tully, Richard O'Kennedy, Tim Gibson, Paul A. Millner, Seamus P.J. Higson, Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications, Biosensors and Bioelectronics, Volume 24, Issue 5, Selected Papers from the Tenth World Congress on Biosensors Shangai, China, May 14-16, 2008, 1 January 2009, Pages 1090-1095
Abstract:
This paper describes the development and characterisation of labeless
immunosensors for (a) the cardiac drug digoxin and (b) bovine serum albumin
(BSA). Commercial screen-printed carbon electrodes were used as the basis for
the sensors. Two methods were used to immobilise antibodies at the electrode
surface. Aniline was electropolymerised onto these electrodes to form a thin
planar film of conductive polyaniline; the polyaniline film was then utilised as
a substrate to immobilise biotinylated anti-digoxin using a classical avidin-
biotin affinity approach. As an alternative approach, poly(1,2-diaminobenzene)
was electrodeposited onto the carbon electrodes and this modified surface was
then sonochemically ablated to form an array of micropores. A second
electropolymerisation step was then used to co-deposit conductive polyaniline
along with antibodies for BSA within these pores to produce a microarray of
polyaniline protrusions with diameters of several μm, containing entrapped anti-
BSA
