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Please use this identifier to cite or link to this item: http://dspace.lib.cranfield.ac.uk/handle/1826/3196

Document Type: Article
Title: Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications.
Authors: Barton, Andrew C.
Collyer, Stuart D.
Davis, Frank
Garifallou, Goulielmos-Zois
Tsekenis, Georgios
Tully, Elizabeth
O'Kennedy, Richard
Gibson, Tim
Millner, Paul A.
Higson, Seamus P. J.
Issue Date: 2009
Citation: Andrew C. Barton, Stuart D. Collyer, Frank Davis, Goulielmos-Zois Garifallou, Georgios Tsekenis, Elizabeth Tully, Richard O'Kennedy, Tim Gibson, Paul A. Millner, Seamus P.J. Higson, Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications, Biosensors and Bioelectronics, Volume 24, Issue 5, Selected Papers from the Tenth World Congress on Biosensors Shangai, China, May 14-16, 2008, 1 January 2009, Pages 1090-1095
Abstract: This paper describes the development and characterisation of labeless immunosensors for (a) the cardiac drug digoxin and (b) bovine serum albumin (BSA). Commercial screen-printed carbon electrodes were used as the basis for the sensors. Two methods were used to immobilise antibodies at the electrode surface. Aniline was electropolymerised onto these electrodes to form a thin planar film of conductive polyaniline; the polyaniline film was then utilised as a substrate to immobilise biotinylated anti-digoxin using a classical avidin- biotin affinity approach. As an alternative approach, poly(1,2-diaminobenzene) was electrodeposited onto the carbon electrodes and this modified surface was then sonochemically ablated to form an array of micropores. A second electropolymerisation step was then used to co-deposit conductive polyaniline along with antibodies for BSA within these pores to produce a microarray of polyaniline protrusions with diameters of several μm, containing entrapped anti- BSA
URI: http://dx.doi.org/10.1016/j.bios.2008.06.001
http://dspace.lib.cranfield.ac.uk/handle/1826/3196
Appears in Collections:Staff publications - Cranfield University at Silsoe

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