Molecular epidemiology and antimicrobial resistance of outbreaks of Klebsiella pneumoniae clinical mastitis in Chinese dairy farms

Date

2022-11-14

Authors

Fu, Shaodong
Wen, Chen
Wang, Zhenglei
Qiu, Yawei
Zhang, Yihao
Zuo, Jiakun
Xu, Yuanyuan
Han, Xiangan
Luo, Zhenhua
Chen, Wei
Miao, Jinfeng

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Journal Title

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Volume Title

Publisher

American Society for Microbiology

Department

Type

Article

ISSN

2165-0497

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Citation

Fu S, Wen C, Wang Z, et al., (2022) Molecular epidemiology and antimicrobial resistance of outbreaks of Klebsiella pneumoniae clinical mastitis in Chinese dairy farms. Microbiology Spectrum, Volume 10, Number 6, December 2022, Article number e02997-22

Abstract

Klebsiella pneumoniae is an opportunistic pathogen that causes serious infections in humans and animals. However, the availability of epidemiological information on clinical mastitis due to K. pneumoniae is limited. To acquire new information regarding K. pneumoniae mastitis, data were mined about K. pneumoniae strains on dairy cattle farms (farms A to H) in 7 Chinese provinces in 2021. Hypermucoviscous strains of K. pneumoniae were obtained by the string test. MICs of antimicrobial agents were determined via the broth microdilution method. Ten antimicrobial resistance genes and virulence genes were identified by PCR. The prevalence of K. pneumoniae was 35.91% (65/181), and 100% of the bacteria were sensitive to enrofloxacin. Nine antimicrobial resistance genes and virulence genes were identified and compared among farms. The hypermucoviscous phenotype was present in 94.44% of isolates from farm B, which may be a function of the rmpA virulence gene. Based on these data, the multidrug-resistant strains SD-14 and HB-21 were chosen and sequenced. Genotypes were assayed for K. pneumoniae isolates from different countries and different hosts using multilocus sequence typing (MLST). Ninety-four sequence types (STs) were found, and 6 STs present a risk for spreading in specific regions. Interestingly, ST43 was observed in bovine isolates for the first time. Our study partially reveals the current distribution characteristics of bovine K. pneumoniae in China and may provide a theoretical basis for the prevention and treatment of bovine K. pneumoniae mastitis.

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Keywords

antimicrobial resistance, bovine clinical mastitis, Klebsiella pneumoniae, molecular epidemiology

Rights

Attribution 4.0 International

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