A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line

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dc.contributor.author Bouzy, Pascaline
dc.contributor.author O’Grady, Shane
dc.contributor.author Madupalli, Honey
dc.contributor.author Tecklenburg, Mary
dc.contributor.author Rogers, Keith
dc.contributor.author Palombo, Francesca
dc.contributor.author Morgan, Maria P.
dc.contributor.author Stone, Nicholas
dc.date.accessioned 2021-07-22T17:08:37Z
dc.date.available 2021-07-22T17:08:37Z
dc.date.issued 2021-06-11
dc.identifier.citation Bouzy P, O’Grady S, Madupalli H, et al., (2021) A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line. Laboratory Investigation, Volume 101, Issue 9, pp. 1267–1280 en_UK
dc.identifier.issn 0023-6837
dc.identifier.uri https://doi.org/10.1038/s41374-021-00619-0
dc.identifier.uri http://dspace.lib.cranfield.ac.uk/handle/1826/16926
dc.description.abstract Microcalcifications are early markers of breast cancer and can provide valuable prognostic information to support clinical decision-making. Current detection of calcifications in breast tissue is based on X-ray mammography, which involves the use of ionizing radiation with potentially detrimental effects, or MRI scans, which have limited spatial resolution. Additionally, these techniques are not capable of discriminating between microcalcifications from benign and malignant lesions. Several studies show that vibrational spectroscopic techniques are capable of discriminating and classifying breast lesions, with a pathology grade based on the chemical composition of the microcalcifications. However, the occurrence of microcalcifications in the breast and the underlying mineralization process are still not fully understood. Using a previously established model of in vitro mineralization, the MDA-MB-231 human breast cancer cell line was induced using two osteogenic agents, inorganic phosphate (Pi) and β-glycerophosphate (βG), and direct monitoring of the mineralization process was conducted using Raman micro-spectroscopy. MDA-MB-231 cells cultured in a medium supplemented with Pi presented more rapid mineralization (by day 3) than cells exposed to βG (by day 11). A redshift of the phosphate stretching peak for cells supplemented with βG revealed the presence of different precursor phases (octacalcium phosphate) during apatite crystal formation. These results demonstrate that Raman micro-spectroscopy is a powerful tool for nondestructive analysis of mineral species and can provide valuable information for evaluating mineralization dynamics and any associated breast cancer progression, if utilized in pathological samples. en_UK
dc.language.iso en en_UK
dc.publisher Nature Publishing Group en_UK
dc.rights Attribution 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.subject Breast cancer en_UK
dc.subject Cancer models en_UK
dc.title A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line en_UK
dc.type Article en_UK

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